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The GoAdV was propagated in goose embryo fibroblasts (GEFs), the morphological properties of the virions were studied by electron microscopy, and the full genome sequence was determined and analyzed. The results revealed that the infected cells became round and clustered like grapes, virions accumulated and were arranged in crystal lattice formations in the nucleus of GEFs with a diameter of \u223c80\u2009nm. The new isolate (named CH\u2010FJZZ\u2010202201) has a viral genome size of 43,480\u2009bp and shared 96.69% sequence identity with GoAdV\u20104 (P29), representing the species <jats:italic>Goose aviadenovirus A<\/jats:italic>. Phylogenetic analysis showed that CH\u2010FJZZ\u2010202201 was in the same genetic evolutionary branch with the viruses of <jats:italic>Aviadenovirus<\/jats:italic> and was the closest relative to GoAdV\u20104 P29\/Hungary. 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