{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T23:49:58Z","timestamp":1775260198383,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,14]],"date-time":"2024-09-14T00:00:00Z","timestamp":1726272000000},"content-version":"vor","delay-in-days":257,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Biomaterials"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Ridge preservation is an important technique for maintaining the dimensions of the alveolar bone following tooth extraction, which is crucial for successful tooth rehabilitation. The combination of bovine amniotic membrane and hydroxyapatite has shown promise as a scaffold material containing growth factors that can stimulate osteogenic\u2010related factors such as bone morphogenetic protein 2 (BMP2), Runt\u2010related transcription factor 2 (RUNX2), and osteocalcin. This stimulation leads to collagen production and osteoblast proliferation, resulting in new bone formation. In this study, bovine amniotic membrane\u2010hydroxyapatite (BAM\u2010HA) composites were prepared using three different ratios of bovine amniotic membrane and hydroxyapatite (2\u2009:\u20093, 3\u2009:\u20097, 7\u2009:\u200913). Thirty <jats:italic>Sprague\u2013Dawley<\/jats:italic> rats had their first incisors extracted, and different types of BAM\u2010HA were applied for ridge preservation. The control group received no treatment, while the positive control group was given xenograft. After 14 and 28\u2009days, the animals were sacrificed, and immunohistochemical analysis was performed to evaluate the expression of BMP2, RUNX2, and osteocalcin. Additionally, a histological examination was conducted to analyse collagen thickness and osteoblast cell proliferation. The results demonstrated that the application of BAM\u2010HA significantly increased collagen density, osteoblast cell proliferation, and the expression of BMP2, RUNX2, and osteoclacin compared to the control group (<jats:italic>p<\/jats:italic> &lt; 0.05) on both days 14 and 28. Furthermore, increasing the hydroxyapatite content in the composite was found to enhance collagen thickness, osteoblast cell proliferation, and the expression of osteogenic\u2010related factors. These preliminary findings suggest that the combination of BAM\u2010HA can be used for ridge preservation to prevent further bone resorption following tooth extraction.<\/jats:p>","DOI":"10.1155\/2024\/4053527","type":"journal-article","created":{"date-parts":[[2024,9,14]],"date-time":"2024-09-14T13:35:00Z","timestamp":1726320900000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Role of Bovine Amniotic Membrane and Hydroxyapatite for the Ridge Preservation"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8764-8365","authenticated-orcid":false,"given":"Octarina","family":"Octarina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1969-2380","authenticated-orcid":false,"given":"Elly","family":"Munadziroh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3755-5950","authenticated-orcid":false,"given":"Fathilah Abdul","family":"Razak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekowati","family":"Handharyani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6052-2287","authenticated-orcid":false,"given":"Meircurius Dwi Condro","family":"Surboyo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,14]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/cid.12623"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034513507581"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1708-8208.2009.00234.x"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/cid.12364"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41415-019-0647-2"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/0885328217739753"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.5005\/jp-journals-10042-1040"},{"key":"e_1_2_11_8_2","article-title":"Bovine freeze dried amniotic membrane (FD-AM) covered sterile gauze for wound dressing","volume":"68","author":"Siswanto R.","year":"2013","journal-title":"Asian Academic Society International Confrence"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1076\/0271-3683(200003)2031-9ft173"},{"key":"e_1_2_11_10_2","article-title":"Properties of the amniontic membrane for potential use in tissue enginering","volume":"88","author":"Niknejad H.","year":"2008","journal-title":"European Cells and Materials"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/cid.12707"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.5005\/jp-journals-10015-2155"},{"key":"e_1_2_11_13_2","first-page":"287","article-title":"Hidroksiapatit dan Aplikasinya di Bidang Kedokteran Gigi","volume":"11","author":"Mozartha M.","year":"2015","journal-title":"Journal of Visual Languages & Computing"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/coatings12101403"},{"key":"e_1_2_11_15_2","first-page":"479","article-title":"Characterization of bovine sponge amnion (BSA) by a novel process for dental treatment","volume":"15","author":"Munadziroh E.","year":"2022","journal-title":"Journal of International Dental and Medical Research"},{"key":"e_1_2_11_16_2","first-page":"114","article-title":"The healing socket and socket regeneration","volume":"29","author":"Steiner G. 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