{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T10:19:09Z","timestamp":1770286749327,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T00:00:00Z","timestamp":1723420800000},"content-version":"vor","delay-in-days":224,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC (81970106)"],"award-info":[{"award-number":["NSFC (81970106)"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells International"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p><jats:italic>Objectives<\/jats:italic>. To explore the efficacy and the mechanism of the umbilical cord\u2010derived cells combined with cyclosporine A (CsA) in treating aplastic anemia (AA) in mice. <jats:italic>Methods<\/jats:italic>. Immune\u2010mediated AA model mice were treated with CsA\u2009+\u2009UC mesenchymal stem cells (UC\u2010MSC), CsA\u2009+\u2009umbilical cord blood regulatory T cells (UCB\u2010T<jats:sub>reg<\/jats:sub>), UC\u2010MSC, UCB\u2010T<jats:sub>reg<\/jats:sub>, CsA alone, or blank control, respectively (<jats:italic>n<\/jats:italic>\u2009=\u20099 mice\/group). CsA and the cell infusion was administered on d0. Routine peripheral blood testing was performed once weekly; bone marrow colony culture, bone marrow cell flow cytometry, peripheral blood T cell subsets, and serum inflammatory cytokines tests were performed on d14. Transcriptome sequencing was performed for cells from CsA\u2009+\u2009UC\u2010MSC, CsA\u2009+\u2009UCB\u2010T<jats:sub>reg<\/jats:sub>, and CsA groups to detect the possible related genes. Gene function cluster and signal pathway enrichment analysis were also performed. <jats:italic>Results<\/jats:italic>. Blank control mice died due to pancytopenia within 21 days, whereas mice in other groups survived for &gt;28 days. On d14, the CsA\u2009+\u2009UC\u2010MSC and CsA\u2009+\u2009UCB\u2010T<jats:sub>reg<\/jats:sub> groups had higher white blood cell (WBC) counts than the other groups (<jats:italic>p<\/jats:italic> &lt; 0.05), along with higher burst\u2010forming unit (BFU) and colony\u2010forming unit\u2010granulocyte, macrophage (CFU\u2010GM) counts (<jats:italic>p<\/jats:italic> &lt; 0.01). The CsA\u2009+\u2009UC\u2010MSC group had the highest BFU count (<jats:italic>p<\/jats:italic> &lt; 0.01). The CsA\u2009+\u2009UC\u2010MSC and CsA\u2009+\u2009UCB\u2010T<jats:sub>reg<\/jats:sub> groups exhibited the highest bone marrow CD34<jats:sup>+<\/jats:sup> cell proportion (9.68%\u2009\u00b1\u20091.35% and 8.17%\u2009\u00b1\u20090.53%, respectively; <jats:italic>p<\/jats:italic> &lt; 0.01). Tumor necrosis factor (TNF)\u2010<jats:italic>\u03b1<\/jats:italic> and interleukin (IL)\u20102 levels in the CsA\u2009+\u2009UC\u2010MSC group (<jats:italic>p<\/jats:italic> &lt; 0.05) and TNF\u2010<jats:italic>\u03b1<\/jats:italic>, interleukin\u20102, and interferon (INF)\u2010<jats:italic>\u03b3<\/jats:italic> levels in the CsA\u2009+\u2009UC\u2010T<jats:sub>reg<\/jats:sub> group (<jats:italic>p<\/jats:italic> &lt; 0.01) were lower than those in the CsA group. Compared with CsA treatment, CsA\u2009+\u2009UC\u2010MSC significantly downregulated the histone methylation pathway (<jats:italic>p<\/jats:italic> &lt; 0.05), whereas CsA\u2009+\u2009UCB\u2010T<jats:sub>reg<\/jats:sub> significantly upregulated energy metabolism processes (<jats:italic>p<\/jats:italic> &lt; 0.05). Treatment with CsA\u2009+\u2009UC\u2010MSC upregulated superoxide dismutase activity compared with CsA\u2009+\u2009UCB\u2010T<jats:sub>reg<\/jats:sub> treatment. <jats:italic>Conclusions<\/jats:italic>. Adding UC\u2010MSC or UCB\u2010T<jats:sub>reg<\/jats:sub> to CsA markedly enhanced the reconstruction of hematopoiesis in AA mice, with UC\u2010MSC eliciting greater efficiency than UCB\u2010T<jats:sub>reg<\/jats:sub>. Accordingly, the addition of these cells could further improve immune abnormalities.<\/jats:p>","DOI":"10.1155\/2024\/4095268","type":"journal-article","created":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T16:19:28Z","timestamp":1723479568000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Umbilical Cord Blood\u2010Derived Cells Can Reconstruct Hematopoiesis in an Aplastic Anemia Animal Model"],"prefix":"10.1155","volume":"2024","author":[{"given":"Zesong","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Ji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8002-6651","authenticated-orcid":false,"given":"Miao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5517-4372","authenticated-orcid":false,"given":"Bing","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,12]]},"reference":[{"key":"e_1_2_12_1_2","first-page":"1","article-title":"Chinese expert consensus on the diagnosis and treatment of aplastic anemia","volume":"38","author":"R. B. C. D. (Anemia) Group","year":"2017","journal-title":"Chinese Journal of Hematology"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2007.1.23"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2006.1.72"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/16078454.2019.1642548"},{"key":"e_1_2_12_5_2","article-title":"Bone marrow-derived mesenchymal stem cells suppress T cell activation without inducing allogeneic anergy","volume":"96","author":"Tse W. T.","year":"2000","journal-title":"Blood"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-01-066258"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-11-390708"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019001347"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.7.4159"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12887-021-02562-x"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2015.09.006"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-1577-2"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2017.5616"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2018.59.5.643"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-07-293795"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2014.03.014"},{"key":"e_1_2_12_17_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-06-653667"},{"key":"e_1_2_12_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2009.02.006"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2013.0363"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2016.12.003"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00611"},{"key":"e_1_2_12_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/iub.2035"},{"key":"e_1_2_12_23_2","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/1798795"},{"key":"e_1_2_12_24_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.615195"},{"key":"e_1_2_12_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2014.225"},{"key":"e_1_2_12_26_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.10.3838"},{"key":"e_1_2_12_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2005.05.006"},{"key":"e_1_2_12_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2013.01.002"},{"key":"e_1_2_12_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-016-2629-7"},{"key":"e_1_2_12_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2012.11.006"},{"key":"e_1_2_12_31_2","doi-asserted-by":"publisher","DOI":"10.2217\/epi.10.44"},{"key":"e_1_2_12_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2011.10646.x"},{"key":"e_1_2_12_33_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019000855"},{"key":"e_1_2_12_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.06.093"},{"key":"e_1_2_12_35_2","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.790990"},{"key":"e_1_2_12_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.3015"},{"key":"e_1_2_12_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2017.12.010"},{"key":"e_1_2_12_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2011.09.010"},{"key":"e_1_2_12_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2017.11.003"},{"key":"e_1_2_12_40_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.723913"},{"key":"e_1_2_12_41_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.HS9.0000969948.37995.d8"}],"container-title":["Stem Cells International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2024\/4095268","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T16:19:36Z","timestamp":1723479576000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2024\/4095268"}},"subtitle":[],"editor":[{"given":"Mahmood-S","family":"Choudhery","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1155\/2024\/4095268"],"URL":"https:\/\/doi.org\/10.1155\/2024\/4095268","archive":["Portico"],"relation":{},"ISSN":["1687-966X","1687-9678"],"issn-type":[{"value":"1687-966X","type":"print"},{"value":"1687-9678","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"2023-12-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"4095268"}}