{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:33:31Z","timestamp":1785760411224,"version":"3.56.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T00:00:00Z","timestamp":1732060800000},"content-version":"vor","delay-in-days":324,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFF0703600"],"award-info":[{"award-number":["2021YFF0703600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Nipah virus (NiV) is an emerging bat\u2010borne zoonotic virus that can be transmitted to humans and other animals through infected bats or contaminated foods. The disease is highly lethal in humans (40%\u201375%) and has the potential for human\u2010to\u2010human transmission. Currently, there are no approved treatments or vaccines for NiV infection in humans or animals. Consequently, there is a pressing need for a highly sensitive, precise, and visually detectable assay to enable early intervention and mitigate the transmission of NiV infection. Here, we report a single\u2010copy sensitive, field\u2010deployable, one\u2010pot visual reverse transcription\u2010recombinase polymerase amplification (RT\u2010RPA)\u2010clustered regularly interspaced short palindromic repeat (CRISPR)\/CRISPR associate system (Cas)12 for the detection of NiV. The assay works by targeting the <jats:italic>N<\/jats:italic> gene of NiV, and the results are directly visible to the naked eye. The assay has demonstrated the ability to detect as few as 5.5\u2009copies\/\u03bcl of positive plasmids or 5.5\u2009\u00d7\u200910<jats:sup>1<\/jats:sup>\u2009copies\/\u03bcl of RNA transcripts when reacted at constant temperature for 40\u2009min. It showed high specificity for NiV and had no cross\u2010reaction with other pathogens, including rabies virus (RABV), Japanese encephalitis virus (JEV), herpes simplex virus type 1 (HSV\u20101), Hendra virus (HeV), and <jats:italic>Streptococcus suis<\/jats:italic> (<jats:italic>S. suis<\/jats:italic>), that can cause clinical symptoms similar to those of NiV infection. Moreover, this assay had a 100% coincidence rate with the reverse transcription quantitative polymerase chain reaction (RT\u2010qPCR) method recommended by the World Organization for Animal Health (WOAH) for the detection of simulated clinical samples, indicating that it has great potential as an ultrasensitive, simple, and portable novel assay for the onsite diagnosis of NiV infection.<\/jats:p>","DOI":"10.1155\/2024\/4118007","type":"journal-article","created":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T10:20:13Z","timestamp":1732098013000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A Single\u2010Copy Sensitive and Field\u2010Deployable One\u2010Pot RT\u2010RPA CRISPR\/Cas12a Assay for the Specific Visual Detection of the Nipah 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