{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T12:12:30Z","timestamp":1792843950366,"version":"4.5.0"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"vor","delay-in-days":81,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p><jats:italic>Borrelia persica<\/jats:italic> is the causative agent of tick\u2010borne relapsing fever in Israel and is prevalent in the Eastern Mediterranean basin and parts of Asia. Infection with <jats:italic>B. persica<\/jats:italic> causes severe illness and potentially life\u2010threatening complications in humans and companion animals. Isolation and <jats:italic>in vitro<\/jats:italic> cultivation of <jats:italic>B. persica<\/jats:italic> in culture medium is difficult and there are only two previous isolates of this spirochete. Here, we describe the first cultivation of <jats:italic>B. persica<\/jats:italic> from an ill dog. Isolation from the dog\u2019s blood was performed with the Pettenkofer\u2010LMU <jats:italic>Bp<\/jats:italic> medium and spirochetes with vibrant motility and cell density of 2\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\/ml were observed in culture by dark\u2010field microscopy 3 days after sampling. The isolate was passaged every 3\u20134 days with cell densities reaching up to 3\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>\/ml achieved over 30 passages. Cryopreservation was made at \u221280\u00b0C without any cryoprotectant additive, and successful growth from thawed culture samples was observed up to 3 months after freezing with repeated freeze and thaw cycles. Generation time during the exponential growth phase was 14.6\u2009hr. Genetic characterization by polymerase chain reaction amplification and DNA sequencing of the flagellin, glycerophosphodiester phosphodiesterase, and <jats:italic>16S rRNA<\/jats:italic> genes indicated that the isolate is a <jats:italic>B. persica<\/jats:italic> genotype I strain associated with human relapsing fever. The new canine isolate would be helpful for research on the pathogenesis of relapsing fever and the new modifications in cultivation and preservation methods may assist in future studies of relapsing fever by simplifying previously reported <jats:italic>in vitro<\/jats:italic> methods.<\/jats:p>","DOI":"10.1155\/2024\/4144123","type":"journal-article","created":{"date-parts":[[2024,3,23]],"date-time":"2024-03-23T02:05:05Z","timestamp":1711159505000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Isolation and Genetic Characterization of the Human Relapsing Fever Spirochete <i>Borrelia persica<\/i> from a Dog with Improved Cultivation Techniques"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2505-5045","authenticated-orcid":false,"given":"Dor","family":"Shwartz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7336-4698","authenticated-orcid":false,"given":"Yaarit","family":"Nachum-Biala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bar","family":"Ben-Shitrit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8382-7182","authenticated-orcid":false,"given":"Reinhard K.","family":"Straubinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7549-1305","authenticated-orcid":false,"given":"Gad","family":"Baneth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,22]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.108"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.50.4.381-400.1986"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/319347"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00098"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/1956311147"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2009.01.009"},{"key":"e_1_2_9_7_2","doi-asserted-by":"crossref","unstructured":"SafdieG. 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