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To identify the most effective dose of filarial r<jats:italic>Bm<\/jats:italic>ALT\u20102 and r<jats:italic>Wb<\/jats:italic>GST alone or in combination against <jats:italic>B. malayi<\/jats:italic> infection <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. <jats:italic>Methods<\/jats:italic>. <jats:italic>Mastomys<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u20095\u20137\/group) received intramuscular (i.m.) injection with three different doses (25, 50, and 100\u2009<jats:italic>\u03bc<\/jats:italic>g) of r<jats:italic>Bm<\/jats:italic>ALT\u20102 or r<jats:italic>Wb<\/jats:italic>GST, either alone or in combination with alum as the adjuvant. Protective immunity was studied by <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> cytotoxicity assay. To evaluate the cellular immune response, splenocyte proliferation and cytokine profile were assessed. <jats:italic>Results<\/jats:italic>. Serological results revealed a substantial (<jats:italic>p<\/jats:italic> &lt; 0.005) induction of IgG1, IgG2a, and IgG3 responses in vaccinated <jats:italic>Mastomys<\/jats:italic>. <jats:italic>Mastomys<\/jats:italic> immunized with 50\u2009<jats:italic>\u03bc<\/jats:italic>g r<jats:italic>Bm<\/jats:italic>ALT\u20102\u2009+\u2009alum induced 79\u201381% killing against the L3 larvae challenge <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> ADCC assay (<jats:italic>p<\/jats:italic> &lt; 0.005); whereas r<jats:italic>Wb<\/jats:italic>GST\u2009+\u2009alum alone or in combination with r<jats:italic>Bm<\/jats:italic>ALT\u20102\u2009+\u2009alum induced 63\u201368% killing (<jats:italic>p<\/jats:italic> &lt; 0.005) <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>. Antigen\u2010specific cytokine profiles of <jats:italic>Mastomys<\/jats:italic> vaccinated with either <jats:italic>Bm<\/jats:italic>ALT\u20102, <jats:italic>Wb<\/jats:italic>GST or a combination showed elevated IL\u201010, IL\u20104, and IFN\u2010<jats:italic>\u03b3<\/jats:italic> levels, signifying both Th1 and Th2 immune response. <jats:italic>Conclusions<\/jats:italic>. These findings suggest that immunization of <jats:italic>Mastomys<\/jats:italic> with a 50\u2009<jats:italic>\u03bc<\/jats:italic>g\/dose of r<jats:italic>Bm<\/jats:italic>ALT\u20102\u2009+\u2009alum four times at a 4\u2010week interval demonstrated considerable protection against <jats:italic>B. malayi<\/jats:italic> infection.<\/jats:p>","DOI":"10.1155\/2024\/4543922","type":"journal-article","created":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T11:06:57Z","timestamp":1721992017000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dose\u2010Dependent Prophylactic Efficacy of Filarial Antigens Glutathione\u2010S\u2010Transferase and Abundant Larval Transcript\u20102 against <i>Brugia malayi<\/i> Challenge in <i>Mastomys<\/i>"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3521-0510","authenticated-orcid":false,"given":"Mohini 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