{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T04:07:13Z","timestamp":1773720433174,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T00:00:00Z","timestamp":1710460800000},"content-version":"vor","delay-in-days":74,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005680","name":"Gauteng Department of Agriculture and Rural Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005680","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009775","name":"Red Meat Research and Development South Africa","doi-asserted-by":"publisher","award":["P10000142"],"award-info":[{"award-number":["P10000142"]}],"id":[{"id":"10.13039\/501100009775","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p><jats:italic>Mycobacterium tuberculosis<\/jats:italic> complex (MTBC) is a group of bacteria responsible for causing tuberculosis in animals and humans. In South Africa (S.A), slaughterhouses are registered by the government and closely inspected and audited for hygienic slaughter practices. Meat inspection to detect lesions has been used for passive surveillance, monitoring, and diagnosis of the disease status. Information on the current status of bovine tuberculosis (bTB) in livestock in the country is limited. Hence, we investigated the occurrence of <jats:italic>Mycobacterium<\/jats:italic> spp. in the tissues of slaughtered livestock and environmental samples in abattoirs in Gauteng province of South Africa (S.A). The cross\u2010sectional study employing random sampling from cattle, pigs, and sheep (with the collection of liver, lung, spleen, and different lymph nodes) irrespective of lesions was carried out in 19 red meat abattoirs. Five hundred animals were sampled, comprising cattle (<jats:italic>n<\/jats:italic>\u2009=\u2009369), pigs (<jats:italic>n<\/jats:italic>\u2009=\u200990), and sheep (<jats:italic>n<\/jats:italic>\u2009=\u200941). Additionally, 19 environmental samples were collected from feedlots, or where animals drink water while awaiting slaughter, to identify mycobacterial species using culture, acid\u2010fast bacteria staining, and polymerase chain reaction (PCR). The Chi\u2010square and Fisher\u2019s Exact tests were used to detect statistically significant differences in the frequency of detection of <jats:italic>Mycobacterium<\/jats:italic> spp. according to the variables investigated (types of tissues, livestock, abattoirs, etc.). The PCR assays detected no MTBC complex species DNA in the bacterial isolates from cattle (<jats:italic>n<\/jats:italic>\u2009=\u200932). Sequence analysis (16S rDNA) of the isolates from eight cattle confirmed only two species, namely <jats:italic>Mycobacterium colombiense<\/jats:italic> (99.81% identity) and <jats:italic>Mycobacterium simiae<\/jats:italic> (99.42% identity). The remaining isolates were identified as members of the <jats:italic>Actinomadura<\/jats:italic> species. From the environmental samples, bacterial isolation was made from three samples, and two could only be identified up to the genus level (<jats:italic>Mycobacterium<\/jats:italic> species) while the remaining isolate was identified as <jats:italic>Mycobacterium senuense<\/jats:italic> (99.22% identity). The study revealed the absence of bovine tuberculosis\u2010causing pathogens in red meat abattoirs of the Gauteng province. Although non\u2010tuberculous <jats:italic>Mycobacteria<\/jats:italic> have been implicated as potentially causing tuberculosis\u2010like diseases in livestock, their occurrence in the current study was found to be low, but the potential to cause disease cannot be ignored.<\/jats:p>","DOI":"10.1155\/2024\/4636652","type":"journal-article","created":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T17:05:08Z","timestamp":1710522308000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Absence of Tuberculosis\u2010Causing <i>Mycobacteria<\/i> from Slaughtered Livestock Tissues and Environmental Samples, Gauteng Province, South Africa"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2443-3874","authenticated-orcid":false,"given":"Vuyokazi","family":"Mareledwane","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9470-9421","authenticated-orcid":false,"given":"Abiodun A.","family":"Adesiyun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2791-3243","authenticated-orcid":false,"given":"Tiny M.","family":"Hlokwe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,15]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.4172\/2168-9784.1000250"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2014.03.011"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063870802000415"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13620-023-00246-9"},{"key":"e_1_2_11_5_2","first-page":"9","article-title":"A study on the prevalence of tuberculosis in cattle at selected abattoirs in Ethiopia","volume":"2","author":"Woldemariam T.","year":"2021","journal-title":"Journal in Research Microbiology"},{"key":"e_1_2_11_6_2","first-page":"162","article-title":"A prevalence study of bovine tuberculosis in cattle in different farming sytems in the Eastern zone of Tanzania Preventative","volume":"57","author":"Shitaye J. 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