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In South America, <jats:italic>Lachesis muta<\/jats:italic> occasionally causes severe human envenomation, with treatment being conditioned to an unspecific antivenom. In this work, we examined the neutralizing ability of <jats:italic>Coutarea hexandra<\/jats:italic> stem bark hydroalcoholic extract (<jats:italic>Ch<\/jats:italic>\u2010E), including the commercial phytochemicals coumarin and quinine, on the neuromuscular blockade induced by <jats:italic>L. m. muta<\/jats:italic> venom in mouse phrenic nerve\u2010diaphragm preparation. Biological assays were performed following conventional myographic technique ex vivo. <jats:italic>Ch<\/jats:italic>\u2010E was phytochemically characterized to detect the presence of coumarin and quinine using analytical methods. <jats:italic>Ch<\/jats:italic>\u2010E and commercial phytochemicals were tested separately or combined under pre\u2010 and post\u2010venom incubation protocols. <jats:italic>Ch<\/jats:italic>\u2010E attenuated the venom\u2010induced neuromuscular blockade only under the pre\u2010venom incubation protocol. Quinine was not detected in <jats:italic>Ch<\/jats:italic>\u2010E. Commercial coumarin and quinine exhibited a concentration\u2010dependent counteracting effect on the venom\u2010induced neuromuscular blockade. The pre\u2010venom incubation protocol showed to be efficient in attenuating the <jats:italic>L. m. muta<\/jats:italic> venom\u2013induced neuromuscular blockade, most likely due to the presence of coumarin derivatives and unknown alkaloids in this extract.<\/jats:p>","DOI":"10.1155\/2024\/4714510","type":"journal-article","created":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T15:07:59Z","timestamp":1731683279000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Protective Action of <i>Coutarea hexandra<\/i> (Rubiaceae) on the Neuromuscular Blockade Induced by <i>Lachesis muta muta<\/i> (Viperidae: Crotalinae) Venom"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6381-9740","authenticated-orcid":false,"given":"Grazielle D.","family":"Pilon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3474-9944","authenticated-orcid":false,"given":"Anna P.","family":"Farias-de-Fran\u00e7a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2481-3327","authenticated-orcid":false,"given":"Nathalia M.","family":"Cantu\u00e1ria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5403-0596","authenticated-orcid":false,"given":"Magali G.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8270-6247","authenticated-orcid":false,"given":"Aline G.","family":"Le\u00e3o-Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0759-5863","authenticated-orcid":false,"given":"Rafael S.","family":"Floriano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2570-9425","authenticated-orcid":false,"given":"Marcio G.","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5517-3791","authenticated-orcid":false,"suffix":"Jr.","given":"Nelson Jorge","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5998-4193","authenticated-orcid":false,"given":"Otto M. 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