{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:16:58Z","timestamp":1788542218051,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T00:00:00Z","timestamp":1713744000000},"content-version":"vor","delay-in-days":112,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005064","name":"Hebei Province Science and Technology Support Program","doi-asserted-by":"publisher","award":["19226629D"],"award-info":[{"award-number":["19226629D"]}],"id":[{"id":"10.13039\/501100005064","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003482","name":"Department of Education of Hebei Province","doi-asserted-by":"publisher","award":["BJ2021055"],"award-info":[{"award-number":["BJ2021055"]}],"id":[{"id":"10.13039\/501100003482","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31802186"],"award-info":[{"award-number":["31802186"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>\n                    <jats:italic>Elizabethkingia miricola<\/jats:italic>\n                    is an emerging nosocomial pathogen responsible for meningitis, sepsis, urinary tract infection, pneumonia, and joint infection in humans. These pathogens were also reported to be causal agents for meningitis\u2010like disease in cultured frogs, which displayed high infectivity, mortality, and significant loss. In July 2023, 10 outbreaks of infectious meningitis\u2010like disease in bullfrogs occurred in Tangshan area. To determine the causal agent, 70 diseased frogs from 10 farms were collected for etiological identification. Gram\u2010negative bacilli were isolated from the brain and liver of sick bullfrogs and identified as members of\n                    <jats:italic>E. miricola<\/jats:italic>\n                    by biochemical characterization and 16S rRNA sequencing analysis. A total of 42 strains of\n                    <jats:italic>E. miricola<\/jats:italic>\n                    were isolated and further determined as the etiological agent by reproducing neurological symptoms and deaths in an artificial infection test. A representative isolate, HBTS\u20101, was picked up for the pathogenicity test, and the data showed that this stain was highly pathogenic to bullfrogs with an LD\n                    <jats:sub>50<\/jats:sub>\n                    of 3.7\u2009\u00d7\u200910\n                    <jats:sup>5<\/jats:sup>\n                    \u2009CFU. Notably, the isolate also showed high pathogenicity to 5\u2010day\u2010old suckling mice, with an LD\n                    <jats:sub>50<\/jats:sub>\n                    of 3.1\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    \u2009CFU, indicating its potential threat to mammals. Moreover, all the 42\n                    <jats:italic>E. miricola<\/jats:italic>\n                    isolates showed resistance to multiple antibotics without an apparent inhibition zone observed in the test, making the choice of antimicrobial therapy challenging. These novel findings prioritized\n                    <jats:italic>E. miricola<\/jats:italic>\n                    as an important zoonotic agent, which may provide a reference for human medicine.\n                  <\/jats:p>","DOI":"10.1155\/2024\/4733320","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T16:20:06Z","timestamp":1713802806000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Outbreaks of\n                    <i>Elizabethkingia miricola<\/i>\n                    Caused Fatal Meningitis\u2010Like Disease in Cultured Bullfrogs"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5430-6148","authenticated-orcid":false,"given":"Rui","family":"An","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9687-0214","authenticated-orcid":false,"given":"Guanxin","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1333-361X","authenticated-orcid":false,"given":"Xinyi","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9740-301X","authenticated-orcid":false,"given":"Lili","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8480-2957","authenticated-orcid":false,"given":"Chunxiao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8731-465X","authenticated-orcid":false,"given":"Yajing","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7003-2772","authenticated-orcid":false,"given":"Yonghui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4410-427X","authenticated-orcid":false,"given":"Tonglei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiumei","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0806-0306","authenticated-orcid":false,"given":"Zhigang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3332-3212","authenticated-orcid":false,"given":"Zhiqiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,22]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2312.170942"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13101"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/03009858231155415"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13761"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13842"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7090295"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879\u2010017\u20102886\u20107"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2022.965939"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2008.07.015"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/acmi.0.000098"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.37201\/req\/081.2019"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idcr.2022.e01573"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2907.230218"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1094050"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab120"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/193855a0"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01731581"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2023.106150"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00730\u201020"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2022.08.072"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/6407524"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfp.2023.100067"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix1052"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241914554"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/aah.10185"}],"container-title":["Transboundary 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