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This study involved the collection of dry blood spots (DBSs) to determine tick\u2010borne pathogens occurring in slaughtered livestock and abattoir workers in Kumasi. This study employed the use of conventional PCR, RT\u2010PCR, and Sanger sequencing to detect and identify the tick\u2010borne pathogens. The resulting data was analysed using Stata version 13. A total of 175 DBSs were collected from goats (76), cattle (54), and sheep (45) in the Kumasi abattoir (130, 74.29%) and Akwatia Line slaughter slab (45, 25.71%). The pathogens identified were mostly bacterial including <jats:italic>Anaplasma capra<\/jats:italic> (9.71%), <jats:italic>Anaplasma phagocytophilum<\/jats:italic> (1.14%), and <jats:italic>Rickettsia aeschlimannii<\/jats:italic> (0.57.%). The only parasite identified was <jats:italic>Theileria ovis<\/jats:italic> (9.14%). A significant association was seen between <jats:italic>A. capra<\/jats:italic> (<jats:italic>p<\/jats:italic> &lt; 0.001) infection and female sheep sampled from the Akwatia Line slaughter slab. Again, there was a significant association between <jats:italic>T. ovis<\/jats:italic> (<jats:italic>p<\/jats:italic> &lt; 0.001) infections and female sheep from the Kumasi abattoir. From the human DBS (63) screened, the pathogens identified were all bacterial including <jats:italic>Coxiella burnetii<\/jats:italic> (1.89%), <jats:italic>Rickettsia africae<\/jats:italic> (1.89%), and <jats:italic>R. aeschlimannii<\/jats:italic> (1.89%). This study reports the first detection of <jats:italic>R. aeschlimannii<\/jats:italic> in livestock as well as the occurrence of the above\u2010mentioned pathogens in humans in Ghana. Animals can serve as amplifying hosts for infectious pathogens; hence, there is an increased risk of infections among the abattoir workers. Continuous surveillance effort is essential, and abattoir workers need to protect themselves from tick bites and infectious tick\u2010borne pathogens.<\/jats:p>","DOI":"10.1155\/2024\/4848451","type":"journal-article","created":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T14:55:16Z","timestamp":1720968916000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular Detection of Tick\u2010Borne Pathogens in Kumasi: With a First Report of Zoonotic Pathogens in Abattoir Workers"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4470-4504","authenticated-orcid":false,"given":"Seth 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