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Thus, the emergence of MRSA clones poses an important threat to human and animal health. This study is aimed at elucidating the genomics insights of a strong biofilm\u2010producing and multidrug\u2010resistant (MDR) <jats:italic>S. aureus<\/jats:italic> MTR_BAU_H1 strain through whole\u2010genome sequencing (WGS). The <jats:italic>S. aureus<\/jats:italic> MTR_BAU_H1 strain was isolated from food handlers\u2019 hand swabs in Bangladesh and phenotypically assessed for antimicrobial susceptibility and biofilm production assays. The isolate was further undergone to high throughput WGS and analysed using different bioinformatics tools to elucidate the genetic diversity, molecular epidemiology, sequence type (ST), antimicrobial resistance, and virulence gene distribution. Phenotypic analyses revealed that the <jats:italic>S. aureus<\/jats:italic> MTR_BAU_H1 strain is a strong biofilm\u2010former and carries both antimicrobial resistance (e.g., methicillin resistance; <jats:italic>mec<\/jats:italic>A, beta\u2010lactam resistance; <jats:italic>bla<\/jats:italic>Z and tetracycline resistance; <jats:italic>tet<\/jats:italic>C) and virulence (e.g., <jats:italic>sea<\/jats:italic>, <jats:italic>tsst<\/jats:italic>, and <jats:italic>PVL<\/jats:italic>) genes. The genome of the <jats:italic>S. aureus<\/jats:italic> MTR_BAU_H1 belonged to ST1930 that possessed three plasmid replicons (e.g., rep16, rep7c, and rep19), seven prophages, and two clustered regularly interspaced short palindromic repeat (CRISPR) arrays of varying sizes. Phylogenetic analysis showed a close evolutionary relationship between the MTR_BAU_H1 genome and other MRSA clones of diverse hosts and demographics. The MTR_BAU_H1 genome harbours 42 antimicrobial resistance genes (ARGs), 128 virulence genes, and 273 SEED subsystems coding for the metabolism of amino acids, carbohydrates, proteins, cofactors, vitamins, minerals, and lipids. This is the first\u2010ever WGS\u2010based study of a strong biofilm\u2010producing and MDR <jats:italic>S. aureus<\/jats:italic> strain isolated from human hand swabs in Bangladesh that unveils new information on the resistomes (ARGs and correlated mechanisms) and virulence potentials that might be linked to staphylococcal pathogenesis in both humans and animals.<\/jats:p>","DOI":"10.1155\/2024\/5516117","type":"journal-article","created":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T08:49:45Z","timestamp":1721465385000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Genomic Insights of a Methicillin\u2010Resistant Biofilm\u2010Producing <i>Staphylococcus aureus<\/i> Strain Isolated From Food Handlers"],"prefix":"10.1155","volume":"2024","author":[{"given":"Fatimah Muhammad","family":"Ballah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4861-0030","authenticated-orcid":false,"given":"M. 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