{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T07:10:53Z","timestamp":1763536253589,"version":"3.41.2"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T00:00:00Z","timestamp":1715904000000},"content-version":"vor","delay-in-days":137,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes Research"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Islet transplantation (ITx) is an established and safe alternative to pancreas transplantation for type 1 diabetes mellitus (T1DM) patients. However, most ITx recipients lose insulin independence by 3 years after ITx due to early graft loss, such that multiple donors are required to achieve insulin independence. In the present study, we investigated whether skeletal myoblast cells could be beneficial for promoting angiogenesis and maintaining the differentiated phenotypes of islets. In vitro experiments showed that the myoblast cells secreted angiogenesis\u2010related cytokines (vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and stromal\u2010derived factor\u20101<jats:italic>\u03b1<\/jats:italic> (SDF\u20101<jats:italic>\u03b1<\/jats:italic>)), contributed to maintenance of differentiated islet phenotypes, and enhanced islet cell insulin secretion capacity. To verify these findings in vivo, we transplanted islets alone or with myoblast cells under the kidney capsule of streptozotocin\u2010induced diabetic mice. Compared with islets alone, the group bearing islets with myoblast cells had a significantly lower average blood glucose level. Histological examination revealed that transplants with islets plus myoblast cells were associated with a significantly larger insulin\u2010positive area and significantly higher number of CD31\u2010positive microvessels compared to islets alone. Furthermore, islets cotransplanted with myoblast cells showed JAK\u2010STAT signaling activation. Our results suggest two possible mechanisms underlying enhancement of islet graft function with myoblast cells cotransplantation: \u201cindirect effects\u201d mediated by angiogenesis and \u201cdirect effects\u201d of myoblast cells on islets via the JAK\u2010STAT cascade. Overall, these findings suggest that skeletal myoblast cells enhance the function of transplanted islets, implying clinical potential for a novel ITx procedure involving myoblast cells for patients with diabetes.<\/jats:p>","DOI":"10.1155\/2024\/5574968","type":"journal-article","created":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T20:50:18Z","timestamp":1715979018000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Skeletal Myoblast Cells Enhance the Function of Transplanted Islets in Diabetic 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