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However, <jats:italic>M. bovis<\/jats:italic>, as an uncommon pathogen of human TB, may interfere with the test results. A comprehensive understanding of phylogenetically informative mutations in the drug resistance genes of <jats:italic>M. bovis<\/jats:italic> is required to distinguish true resistance\u2010conferring mutations. We analyzed 53 drug resistance genes in 165 <jats:italic>M. bovis<\/jats:italic> isolated from humans using whole\u2010genome sequencing data and found that 98.2% (162\/165) of isolates have pyrazinamide intrinsic genotypic resistance, owing to the H57D mutation in the <jats:italic>pncA<\/jats:italic> gene. 12.1% (20\/165) of <jats:italic>M. bovis<\/jats:italic> isolates were resistant to drugs other than pyrazinamide. Furthermore, we discovered 18 phylogenetically informative mutations that differed between <jats:italic>M. bovis<\/jats:italic> and the major lineages 1\u20134 of <jats:italic>M. tuberculosis<\/jats:italic>. Additionally, we reported false\u2010positive ethambutol resistance caused by <jats:italic>M. bovis<\/jats:italic> infection due to the phylogenetically informative mutation <jats:italic>embB<\/jats:italic> E378A. This study is crucial for gaining insights into the genetic characterization and drug resistance of <jats:italic>M. bovis<\/jats:italic> prevalent in humans, as well as contributing to the development of more accurate molecular diagnostic methods and detection tools for drug resistance.<\/jats:p>","DOI":"10.1155\/2024\/5578214","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T12:19:08Z","timestamp":1727353148000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Phylogenetically Informative Mutations in Drug Resistance Genes of Human\u2010Infecting <i>Mycobacterium bovis<\/i>"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2891-8449","authenticated-orcid":false,"given":"Yuhui","family":"Dong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3126-4450","authenticated-orcid":false,"given":"Xichao","family":"Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanzhi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiduo","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoran","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Ge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Nan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7890-4312","authenticated-orcid":false,"given":"Yanlin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1566-5745","authenticated-orcid":false,"given":"Xiangmei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,26]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2018.8"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.129544.111"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(21)00301-3"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00159-19"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30004-5"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-020-00726-5"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30013-6"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30139-6"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30166-3"},{"key":"e_1_2_12_10_2","doi-asserted-by":"crossref","unstructured":"SawyerJ. 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