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As the ARA level increased, the liver and muscle effectively accumulated <jats:italic>n<\/jats:italic>\u22126 polyunsaturated fatty acids (<jats:italic>n<\/jats:italic>\u22126 PUFAs; <jats:italic>p<\/jats:italic> &lt; 0.05). However, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and <jats:italic>n<\/jats:italic>\u22123 PUFA contents of liver and muscle significantly decreased by increasing dietary ARA level (<jats:italic>p<\/jats:italic> &lt; 0.05). Results of liver histology showed dramatically increased vacuolar fat droplets leading to hepatic fat pathological changes in fish fed diets with ARA levels of 1.94% and 2.42% (<jats:italic>p<\/jats:italic> &lt; 0.05). Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities increased with increasing dietary ARA level which was accompanied with elevated liver lipid content (<jats:italic>p<\/jats:italic> &lt; 0.05). Consistently, triglyceride (TG) and nonesterified fatty acid (NEFA) concentrations of serum and liver, and serum cholesterol (CHO) concentration increased (<jats:italic>p<\/jats:italic> &lt; 0.05). As the level of dietary ARA increased, the indicators of lipid metabolism such as sirtuin 1 (<jats:italic>sirt1<\/jats:italic>) and peroxisome proliferator\u2010activated receptor <jats:italic>\u03b1<\/jats:italic> (<jats:italic>ppar\u03b1<\/jats:italic>) also increased (<jats:italic>p<\/jats:italic> &lt; 0.05). However, after reaching their peak in ARA1.04 group, the level of these indicators declined (<jats:italic>p<\/jats:italic> &lt; 0.05). The same trend was observed for the expression of genes related to the downstream pathways. While the mRNA levels of sterol regulatory element\u2013binding protein\u20101 (<jats:italic>srebp-1<\/jats:italic>) and its downstream genes were markedly increased with the increase of dietary ARA level (<jats:italic>p<\/jats:italic> &lt; 0.05). In conclusion, these data suggested that the optimum dietary ARA requirement of <jats:italic>A. schlegelii<\/jats:italic> is 1.03% of diet based on the WG. The study revealed that a diet containing 1.04% ARA can activate the expression levels of <jats:italic>sirt1<\/jats:italic> and <jats:italic>ppar\u03b1<\/jats:italic> leading to promoted lipolysis. However, dietary ARA levels of \u22651.42% induced lipid accumulation in the liver, as they suppressed the mRNA levels of <jats:italic>sirt1<\/jats:italic> and <jats:italic>ppar\u03b1<\/jats:italic>, while elevating the expression level of genes related to lipogenesis.<\/jats:p>","DOI":"10.1155\/2024\/5589032","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T13:34:56Z","timestamp":1731591296000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of the Optimum Dietary Arachidonic Acid Level and Its Essentiality for Black Seabream (<i>Acanthopagrus schlegelii<\/i>): Based on Growth and Lipid Metabolism"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6004-0448","authenticated-orcid":false,"given":"Yangguang","family":"Bao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0559-4955","authenticated-orcid":false,"given":"Yuedong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2094-225X","authenticated-orcid":false,"given":"Wenli","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4748-0921","authenticated-orcid":false,"given":"Bingqian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1709-9290","authenticated-orcid":false,"given":"Xiaoyi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2346-6986","authenticated-orcid":false,"given":"Shunshun","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6513-1851","authenticated-orcid":false,"given":"Peng","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8712-0440","authenticated-orcid":false,"given":"\u00d3scar","family":"Monroig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8075-0885","authenticated-orcid":false,"given":"Qicun","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0188-0292","authenticated-orcid":false,"given":"Min","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,14]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2010.12.024"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2015.01.010"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2016.01.001"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2095.2010.00784.x"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/are.12532"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/bdrb.20311"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2015.07.007"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2014.11.036"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2018.08.015"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-016-4167-4"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-89366-2_13"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114519001946"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/1028415X.2017.1347373"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.294.5548.1871"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2017.11.055"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0143622"},{"key":"e_1_2_12_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2017.11.004"},{"key":"e_1_2_12_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2018.03.005"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114515000781"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/fishes7040185"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2019.734757"},{"key":"e_1_2_12_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.735098"},{"key":"e_1_2_12_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/are.15327"},{"key":"e_1_2_12_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-017-0433-5"},{"key":"e_1_2_12_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2016.05.004"},{"key":"e_1_2_12_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/b135782"},{"key":"e_1_2_12_27_2","volume-title":"Nutrient 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