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A coculture system was set up to actively replicate both viruses, incorporating CD4 T lymphocytes (Jurkat), hepatic stellate cells (LX\u20102), and hepatocytes (Huh7.5). LX\u20102 cells\u2019 susceptibility to HIV infection was assessed through measurements of HIV receptor expression, exposure to cell\u2010free virus, and cell\u2010to\u2010cell contact with HIV\u2010infected Jurkat cells. The study evaluated profibrotic parameters, including programed cell death, ROS imbalance, cytokines (IL\u20106, TGF\u2010<jats:italic>\u03b2<\/jats:italic>, and TNF\u2010<jats:italic>\u03b1<\/jats:italic>), and extracellular matrix components (collagen, <jats:italic>\u03b1<\/jats:italic>\u2010SMA, and MMP\u20109). The impact of HCV infection on LX\u20102\/HIV\u2010Jurkat was examined using soluble factors released from HCV\u2010infected hepatocytes. Despite LX\u20102 cells being nonsusceptible to direct HIV infection, bystander effects were observed, leading to increased oxidative stress and dysregulated profibrotic cytokine release. Coculture with HIV\u2010infected Jurkat cells intensified hepatic fibrosis, redox imbalance, expression of profibrotic cytokines, and extracellular matrix production. Conversely, HCV\u2010infected Huh7.5 cells exhibited elevated profibrotic gene transcriptions but without measurable effects on the LX\u20102\/HIV\u2010Jurkat coculture. This study highlights how HIV\u2010infected lymphocytes worsen hepatic fibrosis during HCV\/HIV coinfection. They increase oxidative stress, profibrotic cytokine levels, and extracellular matrix production in hepatic stellate cells through direct contact and soluble factors. These insights offer valuable potential therapies for coinfected individuals.<\/jats:p>","DOI":"10.1155\/2024\/6343757","type":"journal-article","created":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T01:20:09Z","timestamp":1714526409000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Bystander Effects and Profibrotic Interactions in Hepatic Stellate Cells during HIV and HCV Coinfection"],"prefix":"10.1155","volume":"2024","author":[{"given":"Cintia","family":"Cevallos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricio","family":"Jarmoluk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franco","family":"Sviercz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cinthya A. 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