{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T10:44:28Z","timestamp":1775817868212,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T00:00:00Z","timestamp":1705363200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Brucellosis is a sanitary and economically relevant disease affecting humans, livestock, and wildlife. Ticks have been suggested as vectors, long\u2010term carriers, and amplifiers of <jats:italic>Brucella<\/jats:italic>. In this study, ticks from wildlife ungulate hosts living in hunting reserves of a central region of Spain were collected during a 6\u2010year period, pooled, and screened for <jats:italic>Brucella<\/jats:italic> spp. by PCR. Aiming to correlate <jats:italic>Brucella<\/jats:italic> spp. DNA presence in ticks with <jats:italic>Brucella<\/jats:italic> spp. infections in wildlife ungulate hosts, liver samples from deceased wildlife ungulates coming from the hunting reserves showing a positive result for <jats:italic>Brucella<\/jats:italic> in ticks were tested using a commercial ELISA. In total, 229 tick pools from wild boar (<jats:italic>Sus scrofa<\/jats:italic>, <jats:italic>n<\/jats:italic>\u2009=\u2009176; 76.8%, 95% CI 70.9%\u201381.8%), red deer (<jats:italic>Cervus elaphus<\/jats:italic>, <jats:italic>n<\/jats:italic>\u2009=\u200940; 17.4%, 95% CI 13.1%\u201322.9%), mouflon (<jats:italic>Ovis orientalis musimon<\/jats:italic>, <jats:italic>n<\/jats:italic>\u2009=\u20097; 3.06%, 95% CI 1.49%\u20136.17%), and fallow deer (<jats:italic>Dama dama<\/jats:italic>, <jats:italic>n<\/jats:italic>\u2009=\u20096; 2.62%, 95% CI 1.21%\u20135.60%) were analyzed. PCR results showed that 3.93% (95% CI 2.08%\u20137.30%) tick pools (9\/229) from 16.6% hunting reserves (7\/41) screened yielded a positive PCR result for <jats:italic>Brucella<\/jats:italic>. All positive ticks were <jats:italic>Dermacentor (Dermacentor marginatus<\/jats:italic> or <jats:italic>Dermacentor reticulatus<\/jats:italic>) collected from wild boar. Ticks collected from wild boars were positive to <jats:italic>Brucella<\/jats:italic> in a relative percentage of 5.10% (95% CI\u2009=\u20091.61\u201311.4) in 2018 and of 7.59% (95% CI\u2009=\u20092.79\u201315.6) in 2021 (6\u2010year prevalence of 5.17%, 9\/176). ELISA showed positive results in three wild boars coming from two out of seven hunting reserves (28.5%) with a positive PCR for <jats:italic>Brucella<\/jats:italic> in ticks. To conclude, <jats:italic>Brucella<\/jats:italic> spp. DNA can be detected in <jats:italic>Dermacentor<\/jats:italic> ticks parasitizing wild boars living in hunting reserves harboring <jats:italic>Brucella<\/jats:italic> spp.\u2010seropositive wild boars. This study provides evidence that the contribution of arthropod vectors should be considered in the epidemiology of brucellosis in wildlife.<\/jats:p>","DOI":"10.1155\/2024\/6618287","type":"journal-article","created":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T00:09:51Z","timestamp":1705450191000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Detection of <i>Brucella<\/i> in <i>Dermacentor<\/i> Ticks of Wild Boar with Brucellosis"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4304-2713","authenticated-orcid":false,"given":"Agust\u00edn","family":"Rebollada-Merino","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4578-7508","authenticated-orcid":false,"given":"Irene","family":"Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Duque","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6162-1025","authenticated-orcid":false,"given":"Teresa","family":"Garc\u00eda-Seco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Escacena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8843-0010","authenticated-orcid":false,"given":"Lucas","family":"Dom\u00ednguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9543-313X","authenticated-orcid":false,"given":"Antonio","family":"Rodr\u00edguez-Bertos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7376-4237","authenticated-orcid":false,"given":"Nerea","family":"Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,16]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s42770-022-00831-0"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13377"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.566253"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2019.04.025"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268814003513"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2018.10.013"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.915692"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.en.06.010161.002135"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1134\/S106235901007006X"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciq102"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu575"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.2307\/4591852"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-9-236"},{"key":"e_1_2_9_14_2","first-page":"138","article-title":"Infection and transmission of Brucella by ectoparasites","volume":"8","author":"Tovar R. 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