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Baicalein has been used to treat inflammation\u2010related diseases; nevertheless, its specific mechanism of action is unclear. Therefore, we examined the protective effects of baicalein on lipopolysaccharide\u2010induced damage to AR42J pancreatic acinar cells (PACs) and determined its mechanism of action for protection. <jats:italic>Methods<\/jats:italic>. An <jats:italic>in vitro<\/jats:italic> cell model of acute pancreatitis (AP) was established using lipopolysaccharide (LPS) (1\u2009mg\/L)\u2010induced PACs (AR42J), and the relative survival rate was determined using the 3\u2010(4,5)\u2010dimethylthiahiazo(\u2010z\u2010y1)\u20103,5\u2010di\u2010phenytetrazoliumromide (MTT) technique. Flow cytometry was applied to evaluate the apoptotic rates of AR42J PACs. The RNA and protein expression of miR\u2010224\u20105p, poly ADP\u2010ribose polymerase\u20101 (PARP1), nuclear transcription factor\u2010<jats:italic>\u03ba<\/jats:italic>B65 (NF\u2010<jats:italic>\u03ba<\/jats:italic>B65), phospho\u2010kappa B alpha(p\u2010I<jats:italic>\u03ba<\/jats:italic>B\u2010<jats:italic>\u03b1<\/jats:italic>), interleukin(IL)\u201018R, NOD\u2010like receptor thermal protein domain\u2010associated protein 3 (NLRP3), gasdermin D (GSDMD), apoptosis\u2010associated speck\u2010like protein containing a CARD (ASC), and caspase\u20101 was detected based on the WB and RT\u2010PCR assays. IL\u20101<jats:italic>\u03b2<\/jats:italic>, IL\u20106, IL\u201018, and TNF\u2010<jats:italic>\u03b1<\/jats:italic> expression levels in AR42J cells were measured via ELISA method. The cell morphology was examined using the AO\/EB method. <jats:italic>Results<\/jats:italic>. The experiment confirmed a significant increase in the activity of AR42J cells treated with various doses of baicalein. Moreover, IL\u20101<jats:italic>\u03b2<\/jats:italic>, IL\u20106, TNF\u2010<jats:italic>\u03b1<\/jats:italic>, and IL\u201018 expression levels in AR42J cells were dramatically reduced (<jats:italic>P<\/jats:italic>\u2009 &lt; 0.05), while miR\u2010224\u20105p level was obviously enhanced. The protein and gene expression of PARP1, NF\u2010<jats:italic>\u03ba<\/jats:italic>B65, p\u2010I<jats:italic>\u03ba<\/jats:italic>B\u2010<jats:italic>\u03b1<\/jats:italic>, IL\u201018R, GSDMD, ASC, NLRP3, and caspase\u20101 was obviously decreased (<jats:italic>P<\/jats:italic> &lt; 0.05). Apoptosis in AR42J cells was significantly reduced with significant improvement in cell morphology. <jats:italic>Conclusion<\/jats:italic>. Baicalein may significantly alleviate LPS\u2010induced AR42J PAC damage by inhibiting the inflammatory response and pyroptosis. Its mode of action might be linked to higher miR\u2010224\u20105p expression, which inhibits the PARP1\/NF\u2010<jats:italic>\u03ba<\/jats:italic>B and NLPR3\/ASC\/caspase\u20101\/GSDMD pathways.<\/jats:p>","DOI":"10.1155\/2024\/6618927","type":"journal-article","created":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T14:39:31Z","timestamp":1728571171000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Protective Effects of Baicalein on Lipopolysaccharide\u2010Induced AR42J PACs through Attenuation of Both Inflammation and Pyroptosis via Downregulation of miR\u2010224\u20105p\/PARP1"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3728-2350","authenticated-orcid":false,"given":"Ming-Wei","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Hai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shou-Hong","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De-Qiong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Qiong","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5192-1826","authenticated-orcid":false,"given":"Yang","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,10]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"crossref","unstructured":"FureyC. 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