{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T12:59:47Z","timestamp":1792760387556,"version":"4.5.0"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T00:00:00Z","timestamp":1720656000000},"content-version":"vor","delay-in-days":192,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020247","name":"University of Bisha","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020247","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p><jats:italic>Dorstenia psilurus<\/jats:italic> is a widely used plant spice in traditional African medicine to treat pain\u2010related conditions. However, the anti\u2010inflammatory mechanisms underlying this activity and the main active ingredients of <jats:italic>D. psilurus<\/jats:italic> have not yet been fully characterized. This study aimed to isolate and identify the main active anti\u2010inflammatory constituents of the <jats:italic>D. psilurus<\/jats:italic> extract and to investigate the underlying anti\u2010inflammatory mechanisms in murine macrophages. Chromatographic techniques and spectroscopic data were used for compound isolation and structure elucidation. The Griess reagent method and the ferrous oxidation\u2010xylenol orange assay were used to evaluate the inhibition of NO production and 15\u2010lipoxygenase activity, respectively. Cyclooxygenase activity was assessed using the fluorometric COX activity assay kit, and Th1\/Th2 cytokine measurement was performed using a flow cytometer. The results indicated that the extract and fractions of <jats:italic>D. psilurus<\/jats:italic> inhibit NO production and proliferation of RAW 264.7 macrophage cells. Bioguided fractionation led to the identification of psoralen, a furocoumarin, as the main bioactive anti\u2010inflammatory compound. Psoralen inhibited NO production and 15\u2010lipoxygenase activity and reduced pro\u2010inflammatory Th1 cytokines (IFN\u2010<jats:italic>\u03b3<\/jats:italic>, TNF\u2010<jats:italic>\u03b1<\/jats:italic>, and IL\u20102) while increasing the secretion of anti\u2010inflammatory cytokines (IL\u20104, IL\u20106, and IL\u201010) in activated RAW 264.7 macrophage cells. The encouraging results obtained in this study suggest that psoralen\u2010based multiple modulation strategies could be a useful approach to address the treatment of inflammatory diseases.<\/jats:p>","DOI":"10.1155\/2024\/8233689","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T19:20:00Z","timestamp":1720725600000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Psoralen Isolated from the Roots of <i>Dorstenia psilurus<\/i> Welw. 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