{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T20:33:26Z","timestamp":1779309206067,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T00:00:00Z","timestamp":1724889600000},"content-version":"vor","delay-in-days":241,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002911","name":"Universidad Complutense de Madrid","doi-asserted-by":"publisher","award":["GRFN17\/21"],"award-info":[{"award-number":["GRFN17\/21"]}],"id":[{"id":"10.13039\/501100002911","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>\n                    Leishmaniasis is a chronic global arthropod\u2010borne zoonotic disease produced by several species of\n                    <jats:italic>Leishmania<\/jats:italic>\n                    with cutaneous, mucocutaneous, and visceral clinical manifestations. In Spain, only\n                    <jats:italic>Leishmania infantum<\/jats:italic>\n                    has been reported so far, although other species of\n                    <jats:italic>Leishmania<\/jats:italic>\n                    , such as\n                    <jats:italic>L. tropica<\/jats:italic>\n                    and\n                    <jats:italic>L. major<\/jats:italic>\n                    , are present in surrounding countries. The aim of this work is to analyze the occurrence of\n                    <jats:italic>Leishmania<\/jats:italic>\n                    spp. infection in European wildcats (\n                    <jats:italic>Felis silvestris<\/jats:italic>\n                    ) as sentinels, including their genotypic characterization. Necropsies of 18 road\u2010killed wildcats were conducted. Samples of ear skin and spleen were taken for DNA isolation and PCR of the highly sensitive\n                    <jats:italic>SSU-rDNA<\/jats:italic>\n                    target. Subsequent PCR tests were performed using more specific targets for the determination of\n                    <jats:italic>Leishmania<\/jats:italic>\n                    species:\n                    <jats:italic>hsp70<\/jats:italic>\n                    and\n                    <jats:italic>ITS1<\/jats:italic>\n                    . Positive samples were sequenced, and phylogenetic trees were constructed. Seven wildcats were found positive for\n                    <jats:italic>Leishmania<\/jats:italic>\n                    spp. Based on the\n                    <jats:italic>hsp70<\/jats:italic>\n                    and\n                    <jats:italic>ITS1<\/jats:italic>\n                    sequences, an animal was found to be infected only with\n                    <jats:italic>L. tropica<\/jats:italic>\n                    in ear skin samples, while two cats were found to be infected with\n                    <jats:italic>L. infantum<\/jats:italic>\n                    in both the ear skin and the spleen. In one animal, a clear sequence of\n                    <jats:italic>L. infantum<\/jats:italic>\n                    ITS1 and a sequence of\n                    <jats:italic>L. tropica hsp70<\/jats:italic>\n                    were obtained from the ear skin. Since\n                    <jats:italic>hsp70<\/jats:italic>\n                    and\n                    <jats:italic>ITS1<\/jats:italic>\n                    sequencing was not possible in three cats, the species of\n                    <jats:italic>Leishmania<\/jats:italic>\n                    infecting them was not determined. This is the first report of autochthonous infection with\n                    <jats:italic>L. tropica<\/jats:italic>\n                    in the Iberian Peninsula. Health care professionals, including physicians, dermatologists, and veterinarians, must be aware of this for a correct diagnosis, treatment, and management of possible coinfections.\n                  <\/jats:p>","DOI":"10.1155\/2024\/8259712","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T05:12:43Z","timestamp":1724908363000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Wildlife as a Sentinel for Pathogen Introduction in Nonendemic Areas: First Detection of\n                    <i>Leishmania tropica<\/i>\n                    in Wildlife in Spain"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1074-0347","authenticated-orcid":false,"given":"Iris","family":"Azami-Conesa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4005-4668","authenticated-orcid":false,"given":"Pablo","family":"Matas M\u00e9ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1167-6537","authenticated-orcid":false,"given":"Paula","family":"P\u00e9rez-Moreno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7486-4942","authenticated-orcid":false,"given":"Javier","family":"Carri\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1491-8627","authenticated-orcid":false,"given":"Jos\u00e9 Mar\u00eda","family":"Alunda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0573-4073","authenticated-orcid":false,"given":"Marta","family":"Mateo Barrientos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8104-845X","authenticated-orcid":false,"given":"Mar\u00eda Teresa","family":"G\u00f3mez-Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,29]]},"reference":[{"key":"e_1_2_12_1_2","unstructured":"WHO: health-topics\/leishmaniasis https:\/\/www.who.int\/health-topics\/leishmaniasis#tab=tab_1%5bInternet%5d.WHO:health-topics\/leishmaniasis 2024."},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004349"},{"key":"e_1_2_12_3_2","unstructured":"WHO: neglected diseases leishmaniasis 2023 https:\/\/apps.who.int\/neglected_diseases\/ntddata\/leishmaniasis\/leishmaniasis.html."},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04080-8"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.12877"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9051101"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10030307"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2013.12.001"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2018.03.015"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2018.05.012"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.190334"},{"key":"e_1_2_12_12_2","doi-asserted-by":"crossref","unstructured":"BerriatuaE. P\u00e9rez-CutillasP. Gonz\u00e1lez VidalA. andBri\u00ebtO. The spatial relationship between the presence and absence ofLeishmaniaspp. and leishmaniasis and phlebotomine sand fly vectors in Europe and neighbouring countries 2023 European Centre for Disease Prevention and Control https:\/\/www.ecdc.europa.eu.","DOI":"10.1186\/s13071-024-06484-2"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04081-7"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2019.104069"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(93)90482-6"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2017.05.016"},{"key":"e_1_2_12_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crpvbd.2023.100150"},{"key":"e_1_2_12_18_2","unstructured":"European centre for disease prevention and control and European food safety authority 2023 Phlebotomine sandflies maps [internet]. 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Wildlife as a sentinel for pathogen introduction in nonendemic areas: first detection ofLeishmania tropica in wildlife in Spain 2024 BiorXiv.","DOI":"10.1101\/2024.03.16.585353"}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2024\/8259712","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T09:54:37Z","timestamp":1735898077000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2024\/8259712"}},"subtitle":[],"editor":[{"given":"Nan-hua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":51,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1155\/2024\/8259712"],"URL":"https:\/\/doi.org\/10.1155\/2024\/8259712","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.03.16.585353","asserted-by":"object"}]},"ISSN":["1865-1674","1865-1682"],"issn-type":[{"value":"1865-1674","type":"print"},{"value":"1865-1682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"2024-04-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"8259712"}}