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To evaluate deeper into the anti\u2010inflammatory potential of the specific enantiomer of natural PCB, we conducted the first investigation into the efficacy of the pure enantiomer (2<jats:italic>S<\/jats:italic>)\u2010PCB in modulating inflammatory mediators induced by lipopolysaccharide (LPS) in both murine RAW 264.7 and human U937 macrophage cell lines. This particular compound was isolated from <jats:italic>Goniothalamus macrophyllus<\/jats:italic> (Annonaceae), a native plant of Indonesia. This plant has been used traditionally as an herbal medicine to alleviate inflammation. (2<jats:italic>S<\/jats:italic>)\u2010PCB was isolated from the stem bark of <jats:italic>G. macrophyllus<\/jats:italic> by defatting with <jats:italic>n<\/jats:italic>\u2010hexane followed by maceration with methanol. Purification was performed using several chromatographic techniques. The absolute configuration was determined using electronic circular dichroism (ECD) spectroscopy. This compound was then tested for its inhibitory activity on prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) and subjected to docking simulations. The results indicated that (2<jats:italic>S<\/jats:italic>)\u2010PCB significantly suppressed the production of PGE<jats:sub>2<\/jats:sub> induced by LPS in both RAW 264.7 and U937 cell lines. The docking simulations revealed that (2<jats:italic>S<\/jats:italic>)\u2010PCB reduced PGE<jats:sub>2<\/jats:sub> levels by suppressing mitogen\u2010activated protein kinase (MAPK) activation through inhibiting p38 and extracellular signal\u2010regulated kinases (ERK). These findings suggest that the compound may prevent worsening of septic shock caused by bacterial infection.<\/jats:p>","DOI":"10.1155\/2024\/8811022","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T05:10:30Z","timestamp":1730265030000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Inhibitory Effect of (2<i>S<\/i>)\u2010Pinocembrin From <i>Goniothalamus macrophyllus<\/i> on the Prostaglandin E<sub>2<\/sub> Production in Macrophage Cell Lines"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6363-5239","authenticated-orcid":false,"given":"Hilwan Yuda","family":"Teruna","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6856-9373","authenticated-orcid":false,"given":"Kamal","family":"Rullah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1103-8261","authenticated-orcid":false,"given":"Rudi","family":"Hendra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6554-3987","authenticated-orcid":false,"given":"Rahayu","family":"Utami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2390-9535","authenticated-orcid":false,"given":"Deri","family":"Islami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8043-798X","authenticated-orcid":false,"given":"Siti Munirah","family":"Mohd Faudzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0729-768X","authenticated-orcid":false,"given":"Mohd Fadhlizil Fasihi","family":"Mohd Aluwi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4366-6772","authenticated-orcid":false,"given":"Kok Wai","family":"Lam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,29]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2020.145131"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-172-41548"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307640100"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215528"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0000000000000597"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-012-0342-8"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgp014"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27411"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prostaglandins.2020.106409"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.2217\/pmt.16.4"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.09.015"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.294.5548.1871"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.bor.0000129664.81052.8e"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0802894105"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b01750"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.161.10.5681"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2006.03.021"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2010.12.012"},{"key":"e_1_2_13_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.1.264"},{"key":"e_1_2_13_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.12.4265-4274.2000"},{"key":"e_1_2_13_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11101-014-9367-z"},{"key":"e_1_2_13_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-015-9125-2"},{"key":"e_1_2_13_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2017.10.005"},{"key":"e_1_2_13_24_2","doi-asserted-by":"publisher","DOI":"10.4155\/fmc-2023-0174"},{"key":"e_1_2_13_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(90)80142-4"},{"key":"e_1_2_13_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-94753-8_62-1"},{"key":"e_1_2_13_27_2","unstructured":"DuG. 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