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Most (59%) cases originated from Gauteng province, South Africa. As a follow\u2010up study to the outbreak, we used standard bacteriological and molecular methods to determine the prevalence of pathogenic and virulent serogroups of <jats:italic>L. monocytogenes<\/jats:italic> in various beef and beef products retailed in Gauteng province, South Africa. The overall prevalence of <jats:italic>Listeria<\/jats:italic> spp. was 28% (112\/400), comprising <jats:italic>Listeria monocytogenes<\/jats:italic> (9.3%), <jats:italic>Listeria innocua<\/jats:italic> (16.3%), and <jats:italic>Listeria welshimeri<\/jats:italic> (2.5%) (<jats:italic>p<\/jats:italic> &lt; 0.001). It is crucial to have detected that the region (<jats:italic>p<\/jats:italic> = 0.036), type of product (<jats:italic>p<\/jats:italic> = 0.032), and temperature at storage (<jats:italic>p<\/jats:italic> = 0.011) significantly affected the occurrence of <jats:italic>L. monocytogenes<\/jats:italic> in beef products. It is alarming that pathogenic serogroups 4b\u20104d\u20104e (51.4%) and 1\/2a\u20103a (43.2%) were detected among the isolates of <jats:italic>L. monocytogenes<\/jats:italic>. Importantly, they were all carriers of seven virulence\u2010associated genes (<jats:italic>hlyA, inlB, plcA, iap, inlA, inlC, and inlJ<\/jats:italic>). Our study also demonstrated that 16.7% of \u201cpolony\u201d samples investigated were contaminated with <jats:italic>L. monocytogenes.<\/jats:italic> Considering that pathogenic and virulent <jats:italic>L. monocytogenes<\/jats:italic> contaminated beef and beef products retailed in South Africa, the food safety risk posed to consumers remains and cannot be ignored. Therefore, it is imperative to reduce the contamination of these products with <jats:italic>L. monocytogenes<\/jats:italic> during beef production, processing, and retailing to avoid future outbreaks of human listeriosis in the country.<\/jats:p>","DOI":"10.1155\/2024\/8891963","type":"journal-article","created":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T16:20:09Z","timestamp":1710346809000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Detection of Pathogenic Serogroups and Virulence Genes in <i>Listeria monocytogenes<\/i> Strains Isolated from Beef and Beef Products Retailed in Gauteng Province, South Africa, Using Phenotypic and Polymerase Chain Reaction (PCR)\u2010Based Methods"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9415-5297","authenticated-orcid":false,"given":"James","family":"Gana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nomakorinte","family":"Gcebe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebone","family":"Moerane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf B.","family":"Ngoshe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khomotso","family":"Moabelo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9470-9421","authenticated-orcid":false,"given":"Abiodun A.","family":"Adesiyun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,13]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2016.12.016"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.gpp3-0014-2018"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmmcr.0.003103"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10030512"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2019.04.021"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2017.2419"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.4314\/ejhd.v18i3.9962"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028x.jfp-19-092"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.4102\/ojvr.v87i1.1869"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/jfs.12629"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2006.07.010"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00384"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2021.e06169"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.22099\/ijvr.2020.37618.5472"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1438-4221(00)80086-7"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2004.09.005"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00060-19"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2023.1161486"},{"key":"e_1_2_13_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2017.2419"},{"key":"e_1_2_13_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000144"},{"key":"e_1_2_13_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00925-09"},{"key":"e_1_2_13_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2019.02.007"},{"key":"e_1_2_13_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/genomea.00538-18"},{"key":"e_1_2_13_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/jfs.12732"},{"key":"e_1_2_13_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2019.06.003"},{"key":"e_1_2_13_26_2","volume-title":"The Microbial Ecology of Biltong in South Africa during Production and at point-of-sale","author":"Naidoo K.","year":"2010"},{"key":"e_1_2_13_27_2","article-title":"Microbial quality assessment and predominant microorganism of biltong produced in butcheries in Gaborone, Botswana","volume":"17","author":"Allotey J.","year":"2014","journal-title":"Food and Nutrition Sciences"},{"key":"e_1_2_13_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2013.11.003"},{"key":"e_1_2_13_29_2","first-page":"93","article-title":"Water sorption isotherms of beef biltong and their use in predicting critical moisture contents for biltong storage","volume":"3","author":"Van der Riet W. 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