{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T04:24:55Z","timestamp":1775276695725,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["8196030799"],"award-info":[{"award-number":["8196030799"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangxi Medical and Health Key Cultivation Discipline Construction Project","award":["8196030799"],"award-info":[{"award-number":["8196030799"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2024,4,1]]},"abstract":"<jats:p>Background. Acute pancreatitis (AP) is a clinically frequent acute abdominal condition, which refers to an inflammatory response syndrome of edema, bleeding, and even necrosis caused by abnormal activation of the pancreas\u2019s own digestive enzymes. Intestinal damage can occur early in the course of AP and is manifested by impaired intestinal mucosal barrier function, and inflammatory reactions of the intestinal mucosa, among other factors. It can cause translocation of intestinal bacteria and endotoxins, further aggravating the condition of AP. Therefore, actively protecting the intestinal mucosal barrier, controlling the progression of intestinal inflammation, and improving intestinal dynamics in the early stages of AP play an important role in enhancing the prognosis of AP. Methods. The viability and apoptosis of RAW264.7 cells treated with Esculentoside A (EsA) and\/or lipopolysaccharide were detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry, respectively. The expression of apoptosis-related proteins and NF-\u03baB signaling pathway-related proteins were detected by western blot (WB). An enzyme-linked immunosorbent assay was used to measure TNF-\u03b1 and IL-6 secretion. Results. In vitro experiments demonstrated that EsA not only promoted the apoptosis of inflammatory cells but also reduced the secretion of TNF-\u03b1 and IL-6 in a dose-dependent manner. Additionally, it inhibited the activation of the NF-\u03baB signaling pathway by decreasing the expression of phosphorylated-p65(p-p65) and elevating the expression of I\u03baB\u03b1. Similarly, in vivo experiments using a rat AP model showed that EsA inhibited the expression of p-p65 elevating the expression of I\u03baB\u03b1 in the intestinal tissues of the rat AP model and promoting the apoptosis of inflammatory cells in the intestinal mucosa in vivo experiments, while improving the pathological outcome of the pancreatic and intestinal tissues. Conclusion. Our results suggest that EsA can reduce intestinal inflammation in the rat AP model and that EsA may be a candidate for treating intestinal inflammation in AP and further arresting AP progression.<\/jats:p>","DOI":"10.1155\/2024\/9078794","type":"journal-article","created":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:05:08Z","timestamp":1712016308000},"page":"1-10","source":"Crossref","is-referenced-by-count":4,"title":["Studies Related to the Involvement of EsA in Improving Intestinal Inflammation in Acute Pancreatitis via the NF-\u03baB Pathway"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3871-1967","authenticated-orcid":true,"given":"CuiPing","family":"Pan","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, The Second Affiliated Hospital of Guilin Medical University, Guilin 541100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6497-023X","authenticated-orcid":true,"given":"ChunXiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Second Affiliated Hospital of Guilin Medical University, Guilin 541100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3430-9571","authenticated-orcid":true,"given":"YiJie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Second Affiliated Hospital of Guilin Medical University, Guilin 541100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5370-6207","authenticated-orcid":true,"given":"Jie","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Second Affiliated Hospital of Guilin Medical University, Guilin 541100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5996-8011","authenticated-orcid":true,"given":"ShiWei","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Second Affiliated Hospital of Guilin Medical University, Guilin 541100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0260-1263","authenticated-orcid":true,"given":"HaiCheng","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Second Affiliated Hospital of Guilin Medical University, Guilin 541100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8131-9384","authenticated-orcid":true,"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Second Affiliated Hospital of Guilin Medical University, Guilin 541100, China"},{"name":"Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, The Second Affiliated Hospital of Guilin Medical University, Guilin 541100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.6496"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2017.10.003"},{"issue":"3","key":"3","first-page":"252","article-title":"Relative factors and preventive measures of pancreatic infection in severe acute pancreatitis","volume":"22","author":"S. 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