{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:03:25Z","timestamp":1787162605891,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T00:00:00Z","timestamp":1728432000000},"content-version":"vor","delay-in-days":282,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010699","name":"Monash University Malaysia","doi-asserted-by":"publisher","award":["STG-000048"],"award-info":[{"award-number":["STG-000048"]}],"id":[{"id":"10.13039\/501100010699","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021809","name":"Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia","doi-asserted-by":"publisher","award":["ECR-00034"],"award-info":[{"award-number":["ECR-00034"]}],"id":[{"id":"10.13039\/501100021809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2024,1]]},"abstract":"\n The growing prevalence of commensal antibiotic resistant\n Escherichia coli<\/jats:italic>\n poses a significant concern for the global spread of antibiotic resistance. Stool samples (\n n<\/jats:italic>\n \u2009=\u200935) from a seminomadic indigenous community in Malaysia, the Jehai, were screened for multidrug\u2010resistant bacteria, specifically the extended\u2010spectrum\n \u03b2<\/jats:italic>\n \u2010lactamase (ESBL) producers. Subsequently, whole\u2010genome sequencing was used to provide genomic insights into eight ESBL\u2010producing\n E. coli<\/jats:italic>\n that colonised eight individuals. The ESBL\n E. coli<\/jats:italic>\n isolates carry resistance genes from various antibiotic classes such as the\n \u03b2<\/jats:italic>\n \u2010lactams (\n bla<\/jats:italic>\n TEM<\/jats:sub>\n ,\n bla<\/jats:italic>\n CTX-M\u221215<\/jats:sub>\n and\n bla<\/jats:italic>\n CTX-M\u221255<\/jats:sub>\n ), quinolones (\n gyrA<\/jats:italic>\n ,\n qnrS<\/jats:italic>\n and\n qnrS1<\/jats:italic>\n ) and aminoglycosides (\n aph<\/jats:italic>\n (\n 3\u2032<\/jats:italic>\n )\n -Ia<\/jats:italic>\n ,\n aph<\/jats:italic>\n (\n 6<\/jats:italic>\n )\n -Id<\/jats:italic>\n and\n aac<\/jats:italic>\n (\n 3<\/jats:italic>\n )\n -IId<\/jats:italic>\n ). Three concerning convergence of ESBL, colistin and metal resistance determinants, with three plasmids from H\u2010type lineage harbouring\n bla<\/jats:italic>\n CTX-M<\/jats:sub>\n and\n mcr-1.1<\/jats:italic>\n genes were identified. Using the Oxford Nanopore Technology (ONT) Native Barcoding Kit (SQK\u2010NBD114.24) in conjunction with the R10.4.1 flow cell, which achieved an average read accuracy (\n Q<\/jats:italic>\n \u2009>\u200910) of 99.84%, we further characterised the\n mcr-1.1<\/jats:italic>\n \u2010bearing plasmids, ranging in size from 25 to 28\u2009kb, from three strains of\n E. coli<\/jats:italic>\n . This report represents the first whole genome analysis of multidrug\u2010resistant bacteria, specifically those resistant to colistin, found within the indigenous population in Malaysia. It strongly indicates that the pertinent issue of colistin resistance in the country is far more significant than previously estimated.\n <\/jats:p>","DOI":"10.1155\/2024\/9223696","type":"journal-article","created":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T08:51:24Z","timestamp":1728463884000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Plasmid\u2010Mediated Co\u2010Occurrence of\n mcr-1.1<\/i>\n in Extended\u2010Spectrum\n \u03b2<\/i>\n \u2010Lactamase (ESBL)\u2010Producing\n Escherichia coli<\/i>\n Isolated From the Indigenous Seminomadic Community in Malaysia"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1615-3138","authenticated-orcid":false,"given":"Polly Soo Xi","family":"Yap","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9275-0973","authenticated-orcid":false,"given":"Li-Fang","family":"Yeo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9062-3839","authenticated-orcid":false,"given":"Cindy Shuan Ju","family":"Teh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4541-4819","authenticated-orcid":false,"given":"Amreeta","family":"Dhanoa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8314-068X","authenticated-orcid":false,"given":"Maude Elvira","family":"Phipps","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,9]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.812345"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-17884-8"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gecco.2019.e00815"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.3844\/ajavsp.2019.158.169"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.22038\/IJBMS.2022.61612.13637"},{"key":"e_1_2_12_6_2","unstructured":"Clinical and Laboratory Standards Institute CLSI M100-ED33. 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