{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:21:09Z","timestamp":1753888869574,"version":"3.41.2"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T00:00:00Z","timestamp":1730937600000},"content-version":"vor","delay-in-days":311,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2024J011580","2021J01545"],"award-info":[{"award-number":["2024J011580","2021J01545"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Gastroenterology Research and Practice"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p><jats:bold>Objective:<\/jats:bold> NOTES gallbladder\u2010preserving surgery (N\u2010GPS) has been heralded as a new paradigm shift in minimally invasive surgery for chronic cholecystitis patients. The objective of this research was to evaluate the impact of N\u2010GPS on the intestinal microbiota of patients.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> The study selected patients with benign gallbladder disease (BG group) within 1 week preoperative (BG_DPR stage) and followed up over 1 year postoperative (BG_YPO stage) and selected healthy controls (HC group) whose sex, age, and BMI index matched with patients at BG_YPO stage, too. Accordingly, stool samples from healthy controls and two stages of patients with benign gallbladder disease were collected; among them, the selected samples were sent for 16S rDNA sequencing with Illumina MiSeq platform, and then, the combined samples were sent for short\u2010chain fatty acid (SCFA) analysis with GC\u2010MS platform.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The result of alpha diversity of Shannon index showed that the difference among the two stages of BG group and HC group wasn\u2019t statistically significant, while the result of beta diversity based on the weighted UniFrac distance suggested that the structure of intestinal microbiota of BG group at YPO stage was closer to HC group. LEfSe analysis suggested that BG_YPO stage enriched genus, such as <jats:italic>Enterocloster<\/jats:italic> and <jats:italic>Hungatella_A_128155<\/jats:italic>, which improved bile acid metabolism. Compared with BG_DPR stage, BG_YPO stage and HC group enriched <jats:italic>Faecalibacterium<\/jats:italic> and <jats:italic>Roseburia<\/jats:italic>, but depleted <jats:italic>Streptococcus<\/jats:italic>, while fecal SCFA concentrations increased.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Patients with benign gallbladder disease and chronic cholecystitis after N\u2010GPS treatment for over 1 year improved gut microbial community structure. With the improving bile acid metabolism, SCFA\u2010producing bacteria increased and pathobionts decreased, which helped the intestinal microbiota structure of BG group at YPO stage restore and close to HC group.<\/jats:p><jats:p><jats:bold>Trial Registration:<\/jats:bold> Chinese Clinical Trial Registry identifier: <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/www.chictr.org.cn\/showproj.html?proj=44437\">ChiCTR1900028267<\/jats:ext-link>.<\/jats:p>","DOI":"10.1155\/2024\/9327118","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T10:19:45Z","timestamp":1730974785000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Beneficial Alterations of Intestinal Microbiota in Chronic Cholecystitis Patients Treated With NOTES Gallbladder\u2010Preserving 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