{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T05:52:27Z","timestamp":1791438747359,"version":"4.3.3"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"vor","delay-in-days":182,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1801301"],"award-info":[{"award-number":["2023YFD1801301"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1801302"],"award-info":[{"award-number":["2023YFD1801302"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Rotavirus A (RVA) is a prevalent cause of enteric diarrhea in infants, bovine, pigs, and sheep globally. Currently, the G6P[1]\u2010type rotaviruses are prevalent in sheep or goat in Bangladesh, Turkey, and Uganda. However, this genotype has not been reported in Chinese sheep or goat. Therefore, 12 anal swabs were collected from diarrheal sheep in Gansu Province, China, in 2023 and tested for rotavirus using reverse transcription\u2010polymerase chain reaction (RT\u2010PCR). Pathological sections and immunohistochemistry were used to observe pathological changes and rotavirus antigens in the duodenum, respectively. The sheep rotavirus was isolated in MA\u2010104 cells and characterized through indirect immunofluorescence and transmission electron microscopy. The genes of the strain were obtained using the next\u2010generation sequencing technology and analyzed phylogenetically. One sheep was positive for rotavirus by RT\u2010PCR, and immunohistochemistry revealed numerous rotavirus antigens in the apical portion of the duodenal villi. Transmission electron microscopy revealed that the strain was characterized by virus particles that were \u201cwheel\u2010shaped\u201d and measured 70\u201380\u2009nm in size. The gene constellations of this strain is G6\u2010P[1]\u2010I2\u2010R2\u2010C2\u2010M2\u2010A11\u2010N2\u2010T6\u2010E2\u2010H3. BLASTn and phylogenetic tree analyses suggest that this strain is likely a recombinant of human rotavirus, goat rotavirus, and bovine rotavirus. The comparison of amino acid similarities revealed three differences in the key antigenic epitopes of the VP7 and VP4 proteins between the GO34 strain and this study strain despite the identical gene constellations of the two strains. To date, this is the first report of this constellation of RVA being found in sheep.<\/jats:p>","DOI":"10.1155\/2024\/9614599","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T15:49:40Z","timestamp":1719848980000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Isolation and Genomic Characterization of the G6P[1]\u2010Type Sheep Rotavirus in China"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9149-0939","authenticated-orcid":false,"given":"Ping","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DengShuai","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TianYu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DiXi","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0306-0327","authenticated-orcid":false,"given":"KeShan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183392"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2014.06.016"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.14712"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-008-0155-1"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msq137"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02257-07"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-011-1006-z"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/v14112522"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.12.006"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2011.11.018"},{"key":"e_1_2_11_11_2","unstructured":"LiP. GaiW. ZhaoD. ZhangD. YangX. ShiX. ChenL. ZhaoM. ZhaoS. ChenG. HeW. Y. L. BieX. ShangY. ZhengH. andZhangK. Isolation and molecular characterization of the firstG8-type sheep rotavirus identified in China 2024 Unpublishedhttps:\/\/assets.researchsquare.com\/files\/rs-3788278\/v1\/24330547-73-4b83-8092-ec20c3fc261f.pdf?c=1704192131."},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.022244-0"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.4149\/av_2017_01_77"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2012.08.009"},{"key":"e_1_2_11_15_2","first-page":"170","article-title":"Genotype of rotavirus vaccine strain LLR in China is G10P[15]","volume":"31","author":"Li D.","year":"2015","journal-title":"Chinese Journal of Virology"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1089\/hyb.2012.0016"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2022.100377"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.05.024"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.04.038"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01455-22"},{"key":"e_1_2_11_21_2","article-title":"The origins of G12P[6] rotavirus strains detected in Lebanon","volume":"102","author":"Reslan L.","year":"2020","journal-title":"Journal of General Virology"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-021-01851-y"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2021.104814"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178855"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1170481"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02836"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/s44149-023-00074-y"},{"key":"e_1_2_11_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/s44149-022-00041-z"}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2024\/9614599","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T14:58:56Z","timestamp":1735916336000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2024\/9614599"}},"subtitle":[],"editor":[{"given":"Shao-Lun","family":"Zhai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1155\/2024\/9614599"],"URL":"https:\/\/doi.org\/10.1155\/2024\/9614599","archive":["Portico"],"relation":{},"ISSN":["1865-1674","1865-1682"],"issn-type":[{"value":"1865-1674","type":"print"},{"value":"1865-1682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"2024-01-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"9614599"}}