{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:40:34Z","timestamp":1753886434068,"version":"3.41.2"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:00:00Z","timestamp":1726099200000},"content-version":"vor","delay-in-days":255,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pathogens"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. <jats:italic>Salmonella enterica<\/jats:italic> serovar Typhimurium (<jats:italic>Salmonella<\/jats:italic> Typhimurium) is a common food\u2010borne pathogen that causes gastroenteritis and can lead to life\u2010threatening systemic disease when it spreads to vital organs, such as the liver. Stimulator of interferon genes (STING) is a crucial regulator of the host\u2019s innate immune response to viral infections, while its role in bacterial infections remains controversial. This study aims to establish a <jats:italic>STING<\/jats:italic>\u2010deficient HepG2 cell line through the CRISPR\/Cas9 system and evaluate its effects on <jats:italic>Salmonella<\/jats:italic> replication. <jats:italic>Methods<\/jats:italic>. In this study, a <jats:italic>STING<\/jats:italic> knockout HepG2 cell line was constructed through the application of CRISPR\/Cas9 technology. We assessed cell viability and proliferation using the CCK\u20108 assay. Subsequently, we investigated the effect of <jats:italic>STING<\/jats:italic> deletion on <jats:italic>Salmonella<\/jats:italic> replication and the expression of type I interferon\u2010related genes. <jats:italic>Results<\/jats:italic>. The <jats:italic>STING<\/jats:italic> knockout HepG2 cell line was successfully constructed using the CRISPR\/Cas9 system. The proliferation capability was diminished in <jats:italic>STING<\/jats:italic>\u2010deficient HepG2 cells, while <jats:italic>Salmonella<\/jats:italic> Typhimurium replication in these cells was augmented compared to the wild\u2010type (WT) group. Following <jats:italic>Salmonella<\/jats:italic> infection, the transcriptional responses of type I interferon\u2010related genes, such as <jats:italic>IFNB1<\/jats:italic> and <jats:italic>ISG15<\/jats:italic>, were inhibited in <jats:italic>STING<\/jats:italic>\u2010deficient HepG2 cells. <jats:italic>Conclusions<\/jats:italic>. We successfully constructed a <jats:italic>STING<\/jats:italic>\u2010deficient cell line. Our finding of increased <jats:italic>Salmonella<\/jats:italic> Typhimurium replication in <jats:italic>STING<\/jats:italic>\u2010deficient HepG2 cells provides the basis for further studies on pathogen\u2010host interactions.<\/jats:p>","DOI":"10.1155\/2024\/9615181","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T16:19:16Z","timestamp":1726157956000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of a <i>STING<\/i>\u2010Deficient HepG2 Cell Line through CRISPR\/Cas9 System and Evaluation of Its Effects on <i>Salmonella<\/i> 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