{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T09:21:24Z","timestamp":1793611284648,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T00:00:00Z","timestamp":1704672000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2024,1,8]]},"abstract":"<jats:p>Background. Soft denture lining materials act as a cushion between the denture base and tissues. Alongside having many advantages, its main problem is candida growth due to its rubbery and porous texture. Many interventions have been performed to halt the growth of candida within soft lining materials such as the use of antifungal therapy and strict oral and denture hygiene but there are consequences such as recurrence, drug resistance, and toxicity related to these interventions. Since natural agents such as aloe vera and chitosan have been proven to have antibacterial and antifungal properties with minimum adverse effects, this study aimed to study the effectiveness of chitosan and aloe vera powders incorporated within denture soft lining materials against candida adherence. Methodology. A total of 60 soft-lining material samples were prepared that were equally divided into three groups, viz., group 1 (chitosan incorporation), group 2 (aloe vera incorporation), and group 3 (control). Candida was obtained from the microbiology lab to form a candidal suspension, diluted in 0.9% NaCl to match the McFarland standard bacteriologic solution. Samples were incubated at 37\u00b0C for 24\u2009hours in test tubes containing 100\u2009mL of the candidal suspension and 9.9\u2009mL of the previously prepared Sabouraud dextrose agar. Crystal violet stain was used to stain the adhering cells by fixing them with methanol 80%. For each sample, the adhering candida cells were counted on three standard fields by using an inverted light microscope, and the mean of those fields was recorded. Results. The mean value for samples containing aloe vera was 41.15, while the mean values for samples containing chitosan and the control group were 16.05 and 79.1, respectively. Of all the three groups, aloe vera powder had a significant efficacy against candida growth as compared to the chitosan and control groups (<jats:inline-formula><a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><a:mi>P<\/a:mi><\/a:math><\/jats:inline-formula> value\u2009=\u20090.001). Conclusion. Both herbal agents were effective against candida growth. In comparison, aloe vera was more effective against candida growth compared to chitosan.<\/jats:p>","DOI":"10.1155\/2024\/9918914","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T23:05:08Z","timestamp":1704755108000},"page":"1-6","source":"Crossref","is-referenced-by-count":6,"title":["Effectiveness of Chitosan versus Natural Aloe Vera on Candida Adherence in Denture Soft Lining Material"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8051-9606","authenticated-orcid":true,"given":"Muhammad Rizwan","family":"Memon","sequence":"first","affiliation":[{"name":"Department of Prosthetic Dental Sciences, College of Dentistry, Jouf University, Sakaka, Saudi Arabia"},{"name":"Department of Prosthodontics, Institute of Dentistry, Liaquat University of Medical and Health Sciences, Jamshoro, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1973-1877","authenticated-orcid":true,"given":"Hina","family":"Memon","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Institute of Dentistry, Liaquat University of Medical and Health Sciences, Jamshoro, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5779-4987","authenticated-orcid":true,"given":"Mehwish","family":"Shoro","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Institute of Dentistry, Liaquat University of Medical and Health Sciences, Jamshoro, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1792-6534","authenticated-orcid":true,"given":"Humera","family":"Bhurgri","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Institute of Dentistry, Liaquat University of Medical and Health Sciences, Jamshoro, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0046-3529","authenticated-orcid":true,"given":"Rakhi","family":"Issrani","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry, College of Dentistry, Jouf University, Sakaka, Saudi Arabia"},{"name":"Department of Research Analytics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4769-6653","authenticated-orcid":true,"given":"Azhar","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, College of Dentistry, Jouf University, Sakaka, Saudi 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Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3589-3537","authenticated-orcid":true,"given":"Sherif El Sayed","family":"Sultan","sequence":"additional","affiliation":[{"name":"Department of Prosthetic Dental Sciences, College of Dentistry, Jouf University, Sakaka, Saudi Arabia"},{"name":"Department of Fixed Prosthodontics, College of Dentistry, Tanta University, Tanta, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6258-8132","authenticated-orcid":true,"given":"Hussein Abdelfatth","family":"Ismail","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Zagazig University, Zagazig, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8753-1271","authenticated-orcid":true,"given":"Muhammad Nadeem","family":"Baig","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry, College of 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