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Here, we present a novel infant rat model of mTLE (InfRPil\u2010mTLE) based on a repetitive, triphasic injection regimen consisting of low\u2010dose pilocarpine administrations (180\u2009mg\/kg. i.p.) on days 9, 11, and 15 <jats:italic>post partum<\/jats:italic> (pp). The model had a survival rate of &gt;80% and exhibited characteristic spontaneous recurrent electrographic seizures (SRES) in both the hippocampus and cortex that persisted into adulthood. Using implantable video\u2010EEG radiotelemetry, we quantified a complex set of seizure parameters that demonstrated the induction of chronic electroencephalographic seizure activity in our InfRPil\u2010mTLE model, which predominated during the dark cycle. We further analyzed selected candidate genes potentially relevant to epileptogenesis using a RT\u2010qPCR approach. Several candidates, such as the low\u2010voltage\u2010activated Ca<jats:sup>2+<\/jats:sup> channel Ca<jats:sub>v<\/jats:sub>3.2 and the auxiliary subunits <jats:italic>\u03b2<\/jats:italic><jats:sub>1<\/jats:sub> and <jats:italic>\u03b2<\/jats:italic><jats:sub>2<\/jats:sub>, which were previously reported to be upregulated in the hippocampus of the adult pilocarpine mTLE model, were found to be downregulated (together with Ca<jats:sub>v<\/jats:sub>2.1, Ca<jats:sub>v<\/jats:sub>2.3, M<jats:sub>1<\/jats:sub>, and M<jats:sub>3<\/jats:sub>) in the hippocampus and cortex of our InfRPil\u2010mTLE model. From a translational point of view, our model could serve as a blueprint for childhood epileptic disorders and further contribute to antiepileptic drug research and development in the future.<\/jats:p>","DOI":"10.1155\/2024\/9946769","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T15:41:55Z","timestamp":1721922115000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Novel Rat Infant Model of Medial Temporal Lobe Epilepsy Reveals New Insight into the Molecular Biology and Epileptogenesis in the Developing Brain"],"prefix":"10.1155","volume":"2024","author":[{"given":"Carola","family":"Wormuth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Papazoglou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Henseler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Ehninger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl","family":"Broich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Britta","family":"Haenisch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Hescheler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00fcdiger","family":"K\u00f6hling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6058-4752","authenticated-orcid":false,"given":"Marco","family":"Weiergr\u00e4ber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,25]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2008.04.019"},{"key":"e_1_2_11_2_2","first-page":"473","article-title":"Laboratory animal models of temporal lobe epilepsy","volume":"54","author":"Buckmaster P. 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