{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T08:42:20Z","timestamp":1784796140683,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T00:00:00Z","timestamp":1710892800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["109917\/Z\/15\/Z"],"award-info":[{"award-number":["109917\/Z\/15\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology Research"],"published-print":{"date-parts":[[2024,3,20]]},"abstract":"<jats:p>Background. Natural medicinal products are commonly used as a remedy against malaria infections in African populations and have become a major source of information for the screening of new and more effective antiplasmodial molecules. Therefore, in vitro studies are needed to validate the efficacy of these medicinal products and to explore the potential effects of such drugs on the genetic diversity of Plasmodium falciparum. The current study has investigated the impact of some Beninese plant extracts with antiplasmodial activity on the genetic diversity of P. falciparum. Method. Five (5) ethanolic plant extracts (Dissotis rotundifolia, Ehretia cymosa Thonn, Hibiscus surattensis L., Cola millenii K. Shum, and Costus afer Ker Gawl) and a compound extracted from Ehretia cymosa Thonn (encoded CpE2) were tested against asexual stage parasites of a culture-adapted strain of P. falciparum. Subsequently, the P. falciparum Msp1 and Msp2 markers were genotyped, and the number of allelic variants and the multiplicity of infection (MOI) were compared between drug-exposed and unexposed parasites. Results. All plant extracts have shown inhibitory activity against asexual P. falciparum and selected new allelic variants of the Msp1 and Msp2 genes compared to unexposed parasites. The newly selected allelic variants were K1_100bp and RO33_300bp of the Msp1 gene and FC27_150bp, FC27_300bp, FC27_400bp, and FC27_600bp of the Msp2 gene. However, there was no significant difference in MOI between drug-exposed and unexposed parasites. Conclusion. Our study highlights a source for the selection of new Msp1 and Msp2 alleles after exposure to antimalarial drugs. These findings pave the way for further studies investigating the true roles of these newly selected alleles in P. falciparum.<\/jats:p>","DOI":"10.1155\/2024\/9980715","type":"journal-article","created":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T21:50:34Z","timestamp":1710971434000},"page":"1-8","source":"Crossref","is-referenced-by-count":2,"title":["Beninese Plant Extracts with Antiplasmodial Activity Select New Allele Variants Msp1 and Msp2 in Plasmodium falciparum"],"prefix":"10.1155","volume":"2024","author":[{"given":"Hamirath O.","family":"Lagnika","sequence":"first","affiliation":[{"name":"Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526 Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3091-7456","authenticated-orcid":true,"given":"Adand\u00e9 A.","family":"Medjigbodo","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526 Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1571-1265","authenticated-orcid":true,"given":"Oswald Y.","family":"Djihinto","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526 Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helga M.","family":"Sa\u00efzonou","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526 Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wassiyath A.","family":"Mousse","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526 Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romaric","family":"Akoton","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526 Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurette","family":"Djossou","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526 Cotonou, Benin"},{"name":"Regional Institute of Public Health\/University of Abomey-Calavi, BP 384 Ouidah, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris N.","family":"Vodounkpe","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526 Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Latifou","family":"Lagnika","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Bioactive Natural Substances, Unit of Biochemistry and Molecular Biology, University of Abomey-Calavi, 04 BP, 0320 Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc S.","family":"Djogb\u00e9nou","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526 Cotonou, Benin"},{"name":"Regional Institute of Public Health\/University of Abomey-Calavi, BP 384 Ouidah, Benin"},{"name":"Department of Vector Biology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","author":"WHO World Malaria Report","year":"2023"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S405668"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.medcle.2020.05.024"},{"key":"4","article-title":"World malaria report 2018- World|Relief Web","author":"WHO"},{"key":"5","article-title":"World malaria report","author":"WHO","year":"2021"},{"key":"6","article-title":"WHO recommends R21\/Matrix-M vaccine for malaria prevention in updated advice on immunization","author":"WHO"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-020-03231-7"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6628"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3390\/ph15040470"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1663\/0013-0001(2004)58[S239:MPUTTM]2.0.CO;2"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.9734\/jalsi\/2020\/v23i530161"},{"key":"12","article-title":"Antiplasmodial, antioxidant, hemolytic activities and acute toxicity of Costus Afer Ker Gawl (Costaceae) used in malaria healing in Benin","author":"G. Tiko"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.9734\/ijbcrr\/2020\/v29i530191"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.22270\/jddt.v10i5-s.4491"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-022-04115-8"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2008-9-12-r171"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01291-23"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052746"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-14-492"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-019-3026-1"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1126\/science.781840"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01868-12"},{"key":"23","article-title":"Strain of Plasmodium falciparum (named Ben229) developed in Benin, which will serve for evaluating the efficacy of anti-malarial vaccines and drugs under the conditions in Benin and Africa","author":"L. Djogbenou","year":"2023"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.4236\/ojcd.2013.33020"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(78)90365-3"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.3390\/molecules14083037"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1416"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(93)90131-X"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.12-0056"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-021-07399-y"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis736"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1002\/sim.5706"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00590-19"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2007.12.008"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2001.00761.x"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1995.1096"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-021-04007-3"}],"container-title":["Journal of Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jpr\/2024\/9980715.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jpr\/2024\/9980715.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jpr\/2024\/9980715.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T21:50:37Z","timestamp":1710971437000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jpr\/2024\/9980715\/"}},"subtitle":[],"editor":[{"given":"Bernard","family":"Marchand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,3,20]]},"references-count":37,"alternative-id":["9980715","9980715"],"URL":"https:\/\/doi.org\/10.1155\/2024\/9980715","relation":{},"ISSN":["2090-0031","2090-0023"],"issn-type":[{"value":"2090-0031","type":"electronic"},{"value":"2090-0023","type":"print"}],"subject":[],"published":{"date-parts":[[2024,3,20]]}}}