{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:19:31Z","timestamp":1753888771311,"version":"3.41.2"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T00:00:00Z","timestamp":1709596800000},"content-version":"vor","delay-in-days":64,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["21\/10599-3"],"award-info":[{"award-number":["21\/10599-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.839646\/2023-00"],"award-info":[{"award-number":["88887.839646\/2023-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["307915\/2022-0","312066\/2019-8"],"award-info":[{"award-number":["307915\/2022-0","312066\/2019-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100030732","name":"MSD","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100030732","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004319","name":"Pfizer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004319","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004337","name":"Roche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004337","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>The continued worldwide increase in pet ownership has significantly boosted the growth of the pet food industry accompanied by new food safety risks and challenges. This study was designed to determine the occurrence and molecularly characterize multidrug\u2010resistant (MDR) <jats:italic>Enterobacterales<\/jats:italic> in pet food. Eighty\u2010six (86) packages of dry and wet pet food purchased in different retail stores were screened for carbapenem\u2010resistant <jats:italic>Enterobacterales<\/jats:italic> (CRE). Antimicrobial susceptibility testing was performed by agar dilution technique using EUCAST\/BrCAST recommendations. Blue\u2010Carba test was further used to screen for carbapenemase\u2010producing isolates. Isolated CRE strains were identified at the species level using matrix\u2010assisted laser desorption\/ionisation time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF MS). Detection of carbapenemase\u2010encoding genes was carried out by PCR, Sanger sequencing, and whole genome sequencing (WGS). A total of 15 (17.4%) MDR\u2010CRE (<jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u20092), <jats:italic>Enterobacter cloacae<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u200910), <jats:italic>Leclercia adecarboxylata<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u20092), and <jats:italic>Cronobacter<\/jats:italic> spp. (<jats:italic>n<\/jats:italic>\u2009=\u20091)) were isolated from 86 pet food samples. In addition to being resistant to beta\u2010lactams, the Gram\u2010negative bacterial isolates were also resistant to aminoglycosides, fluoroquinolones, and tigecycline. Interestingly, two carbapenem\u2010resistant <jats:italic>E. coli<\/jats:italic> isolates harboured <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub> gene. WGS analysis of the two <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub>\u2010producing <jats:italic>E. coli<\/jats:italic> isolates revealed that they both belong to ST648 and serotype O153:H2 group. The genetic context of the <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub> showed that they were carried by an IncN plasmid on a Tn<jats:italic>4401b<\/jats:italic> transposon element. To the best of our knowledge, this is the first description of <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub>\u2010harbouring <jats:italic>E. coli<\/jats:italic> ST648 pathogens in pet food. The detection of <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub>\u2010harbouring <jats:italic>E. coli<\/jats:italic> ST648 pandemic high\u2010risk lineage in pet food is worrisome and a serious \u201cOne Health\u201d issue. Therefore, pet food should be considered as a potential vehicle for the transmission of MDR pathogens to companion animals, and a risk factor for the dissemination of these bacterial pathogens to pet animals and their human guardians.<\/jats:p>","DOI":"10.1155\/2024\/9995914","type":"journal-article","created":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T20:50:08Z","timestamp":1709671808000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First Detection of International High\u2010Risk <i>bla<\/i><sub>KPC-2<\/sub>\u2010Harbouring <i>Escherichia coli<\/i> Pandemic Lineage ST648 in Pet Food Packages"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5928-8198","authenticated-orcid":false,"given":"Ikechukwu Benjamin","family":"Moses","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4997-0505","authenticated-orcid":false,"given":"\u00c1ghata Cardoso da Silva","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3956-1599","authenticated-orcid":false,"given":"Tiago Barcelos","family":"Valiatti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5901-0233","authenticated-orcid":false,"given":"Fernanda Fernandes","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0709-6282","authenticated-orcid":false,"given":"Rodrigo","family":"Cay\u00f4","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0913-768X","authenticated-orcid":false,"given":"Ana Cristina","family":"Gales","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,5]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.01125-20"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2021.109284"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.5803\/jsfm.38.67"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02724-0"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw282"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11010048"},{"key":"e_1_2_9_7_2","unstructured":"World Health Organization Global priority list of antibiotic-resistant bacteria to guide research discovery and development of new antibiotics 2017 https:\/\/www.who.int\/medicines\/publications\/WHO-PPL-Short_Summary_25Feb-ET_NM_WHO.pdf."},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkac041"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9090687"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjm.2016.10.002"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz824"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03185-14"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2014.1748"},{"key":"e_1_2_9_14_2","first-page":"1","article-title":"Commission regulation (EU) No 142\/2011 of 25 February 2011 implementing regulation (EC) No 1069\/2009 of the european parliament and of the council laying down health rules as regards animal by-products and derived products not intended for human consumption, L54","author":"Commission","year":"2011","journal-title":"Journal of the European Union"},{"key":"e_1_2_9_15_2","first-page":"42526","article-title":"Compliance policy guide sec. 690.800 Salmonella in food for animals","volume":"78","author":"FDA","year":"2013","journal-title":"Federal Register"},{"key":"e_1_2_9_16_2","unstructured":"EUCAST Breakpoint tables for interpretation of MICs and zone diameters version 12.0 2022 2022 Available online: (Accessed on 20 September)http:\/\/www.eucast.org."},{"key":"e_1_2_9_17_2","unstructured":"BRCast Brazilian committee on antimicrobial susceptibility testing tabela pontos de CorteClinicos-BrCAST 2022 Available online: (Accessed on 20 August).https:\/\/brcast.org.br\/wp-content\/uploads\/2022\/09\/Tabela-pontos-de-corte-clinicos-BrCAST-12-abr-22.pdf."},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01634-13"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.01.004"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2009.10.014"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.02413-12"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0072-8"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.743468"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01265"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2018.09.009"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2020.106040"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12050926"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01743-20"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkac114"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00276"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00116-14"},{"key":"e_1_2_9_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.14223"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.02.014"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.191170"},{"key":"e_1_2_9_36_2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638718823046"},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0187239"},{"key":"e_1_2_9_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx146"},{"key":"e_1_2_9_39_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2016.0246"},{"key":"e_1_2_9_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2018.02.020"},{"key":"e_1_2_9_41_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/6915736"},{"key":"e_1_2_9_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2017.07.011"},{"key":"e_1_2_9_43_2","article-title":"Animal & veterinary","author":"FDA","year":"2021","journal-title":"Outbreaks and Advisories"}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/tbed\/2024\/9995914.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tbed\/2024\/9995914.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2024\/9995914","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T15:00:39Z","timestamp":1735916439000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2024\/9995914"}},"subtitle":[],"editor":[{"given":"Mohsin","family":"Khurshid","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1155\/2024\/9995914"],"URL":"https:\/\/doi.org\/10.1155\/2024\/9995914","archive":["Portico"],"relation":{},"ISSN":["1865-1674","1865-1682"],"issn-type":[{"type":"print","value":"1865-1674"},{"type":"electronic","value":"1865-1682"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"2023-11-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"9995914"}}