{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T03:07:20Z","timestamp":1776481640531,"version":"3.51.2"},"reference-count":75,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T00:00:00Z","timestamp":1754611200000},"content-version":"vor","delay-in-days":219,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005790","name":"Thammasat University","doi-asserted-by":"publisher","award":["TP.2\u201301\/2567"],"award-info":[{"award-number":["TP.2\u201301\/2567"]}],"id":[{"id":"10.13039\/501100005790","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010034","name":"Walailak University","doi-asserted-by":"publisher","award":["WU-IRG-64-067"],"award-info":[{"award-number":["WU-IRG-64-067"]}],"id":[{"id":"10.13039\/501100010034","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advances in Pharmacological and Pharmaceutical Sciences"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:italic>Ardisia polycephala, Iresine herbstii,<\/jats:italic>\n                    and\n                    <jats:italic>Oenanthe javanica<\/jats:italic>\n                    are commonly used to nourish women\u2019s blood and treat reproductive system diseases, according to Thai Traditional Medicine (TTM) scriptures. This study explores the biological activities of these herbs as documented in TTMs. Both water and ethanol extracts of the three herbs were examined for their antioxidant activities using DPPH, FRAP, and NO assays. Additionally, their antiestrogen and cytotoxic effects were investigated, focusing on breast cancer cell lines (T47D and MCF\u20107). Chemical analysis was conducted using the GC\u2013MS technique, with reference data obtained from the National Institute of Standards and Technology (NIST) library. All herbs demonstrated good antioxidant activity. The water extract of\n                    <jats:italic>Ardisia polycephala<\/jats:italic>\n                    (AP\n                    <jats:sup>\u2217\u2217<\/jats:sup>\n                    ) exhibited the highest activity in the DPPH assay (EC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u20097.09\u2009\u00b1\u20091.47\u2009\u03bcg\/mL), which correlated with the FRAP results (358.52\u2009\u00b1\u20091.07\u2009mg Trolox equivalent per gram). The ethanol extract of\n                    <jats:italic>Oenanthe javanica<\/jats:italic>\n                    (OJ\n                    <jats:sup>\u2217<\/jats:sup>\n                    ) specifically showed cytotoxicity against both T47D and MCF\u20107 cell lines (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u200938.06\u2009\u00b1\u20091.52 and 39.93\u2009\u00b1\u20090.36\u2009\u03bcg\/mL, respectively) and demonstrated antiestrogen activity by inhibiting the growth of T47D cells by 99% at 1.3\u2009\u03bcg\/mL when stimulated with 100 pM estradiol. GC\u2013MS analysis identified numerous compounds that support the observed biological activities, including antioxidant and cytotoxic effects. In summary, the three herbs demonstrated antioxidant, cytotoxic, and antiestrogen activities consistent with the chemical compounds identified in the GC\u2013MS analysis. These findings suggest the potential use of these herbs in future breast cancer treatments.\n                  <\/jats:p>","DOI":"10.1155\/adpp\/1234439","type":"journal-article","created":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T05:49:47Z","timestamp":1754718587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Vitro Investigation of the Cytotoxic and Antioxidant Activities of\n                    <i>Ardisia polycephala, Iresine herbstii,<\/i>\n                    and\n                    <i>Oenanthe javanica<\/i>\n                    Extracts With Potential Applications in Breast Cancer Therapy"],"prefix":"10.1155","volume":"2025","author":[{"given":"Nuntika","family":"Prommee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ubonwan","family":"Saesiw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9342-1768","authenticated-orcid":false,"given":"Thana","family":"Juckmeta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pakakrong","family":"Thongdeeying","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kitiya","family":"Yangthaworn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhanuz","family":"Dechayont","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jitpisute","family":"Chunthorng-Orn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pathompong","family":"Phuaklee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tassanee","family":"Ongtanasup","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7366-8771","authenticated-orcid":false,"given":"Onmanee","family":"Prajuabjinda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,8]]},"reference":[{"key":"e_1_2_12_1_2","volume-title":"Local Herbs","author":"Boonyapraphatsara N.","year":"1999"},{"key":"e_1_2_12_2_2","volume-title":"Plant Diversity of Assam. A Checklist of Angiosperms and Gymnosperms","author":"Barooah C.","year":"2014"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2004.01.010"},{"key":"e_1_2_12_4_2","first-page":"1","article-title":"Antimicrobial and Antioxidant Activities of Thai Local Fruit Extracts; Application of a Selected Fruit Extract, Phyllanthus emblica Linn. as a Natural Preservative in Raw Ground Pork During Refrigerated Storage","volume":"2","author":"Nanasombat S.","year":"2012","journal-title":"The Online Journal of Science and Technology (TOJSAT)"},{"key":"e_1_2_12_5_2","first-page":"181","article-title":"Manual for Designating Areas for the Promotion of Medicinal Herb Cultivation for Use in Thai Pharmacology","volume":"1","author":"Department of Thai Traditional and Alternative Medicine","year":"2015","journal-title":"Ministry of Public Health"},{"key":"e_1_2_12_6_2","unstructured":"JongratanavitK.andTongchitpakdeeS. 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