{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:22:30Z","timestamp":1787163750873,"version":"build-2736575974"},"reference-count":100,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T00:00:00Z","timestamp":1740700800000},"content-version":"vor","delay-in-days":58,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/http:\/\/dx.doi.org\/10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AT013164"],"award-info":[{"award-number":["R21AT013164"]}],"id":[{"id":"10.13039\/http:\/\/dx.doi.org\/10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advances in Pharmacological and Pharmaceutical Sciences"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Rationale and Objective:<\/jats:bold>\n                    The pain\u2013depression dyad is highly prevalent and has reciprocal psychological and behavioral effects, leading to poor quality of life, increased disability, and challenging therapeutic outcomes. In an attempt to find better substances that can target pain\u2013depression comorbidity, we examined the effect of aqueous (AE) and ethanol (EE) extracts from\n                    <jats:italic>Acacia sieberiana<\/jats:italic>\n                    (\n                    <jats:italic>A. sieberiana<\/jats:italic>\n                    ) stem bark on reserpinized mice (female and male Swiss albino mice aged 2\u20103\u2009months).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    The dyad was induced with 3 injections (Days 1\u20133) of reserpine (1\u2009mg\/kg\/day,\n                    <jats:italic>s.c<\/jats:italic>\n                    .). Then, animals were treated (Days 4\u20138) with plant extracts (25, 50 and 100\u2009mg\/kg\/day,\n                    <jats:italic>p.o<\/jats:italic>\n                    .) or L\u2010tryptophane (100\u2009mg\/kg\/day,\n                    <jats:italic>i.p<\/jats:italic>\n                    .). Pain\u2010like (tactile and cold allodynia) and depression\u2010like (pole, tail suspension, and force swimming tests) behavioral parameters were evaluated on Days 4 and 8. On Day 9, animals were sacrificed for the quantification of acetylcholinesterase activity, oxidative stress parameters, total catecholamines, dopamine, serotonin, IL\u20101\u03b2, and TNF\u2010\u03b1 levels in the brain or spinal cord. IL\u20101\u03b2 and TNF\u2010\u03b1 were also assayed in the serum. The acute toxicity and phytochemical analysis of EE were conducted.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Reserpine\u2010induced tactile and cold allodynia, depression\u2010like behavior, increased serum IL\u20101\u03b2 and TNF\u2010\u03b1, brain acetylcholinesterase activity, and decreased catecholamine concentration were all reversed by AE and EE. Plant extracts significantly increased dopamine levels and reduced oxidative stress in the brain and\/or spinal cord. No significant effect was observed on brain serotonin and TNF\u2010\u03b1. EE elicited the best pharmacological activity and was nontoxic. LC\u2013MS\/MS molecular networking phytochemical analysis identified 5 compounds with high certainty including piperine, aurantiamide acetate, and asperphenamate.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    AE and EE are effective against pain and depression. Their pharmacological activities might be related to the modulation of inflammation, oxidative stress and catecholamine, and the presence of bioactive natural products.\n                  <\/jats:p>","DOI":"10.1155\/adpp\/1244498","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T10:05:18Z","timestamp":1741687518000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Aqueous and Ethanol Extracts of\n                    <i>Acacia sieberiana<\/i>\n                    (Fabaceae) Stem Bark Reverse the Pain\u2013Depression Dyad in Mice Through Modulation of Catecholamines, Proinflammatory Cytokines, and Oxidative Stress"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-6109-5259","authenticated-orcid":false,"given":"Sorelle Ngassam","family":"Mbankou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2440-3627","authenticated-orcid":false,"given":"Aliance Romain","family":"Fokoua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1634-0624","authenticated-orcid":false,"given":"Cedric Wamba","family":"Koho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2351-1079","authenticated-orcid":false,"given":"Roger Hermann Sadie","family":"Foguieng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6193-8137","authenticated-orcid":false,"given":"Sahar Mofidi","family":"Tabatabaei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9301-4556","authenticated-orcid":false,"given":"Pamela Arielle","family":"Nono Nankam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0501-2604","authenticated-orcid":false,"given":"Kevin Joseph","family":"Tidgewell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5006-6927","authenticated-orcid":false,"given":"T\u00e9lesphore Beno\u00eet","family":"Nguelefack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,28]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000001939"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000002557"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.4415\/ANN_16_02_09"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23841"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaac.2010.05.013"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells10061283"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2022.103311"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s43076-021-00116-9"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jad.2017.05.003"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-021-00513-0"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40122-021-00244-1"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2022.101010"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.0c04739"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.5585"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/9724371"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11011-020-00604-4"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/hup.618"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-021-00638-3"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psyneuen.2011.04.012"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-020-00933-z"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2008.11.029"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.12740\/PP\/76863"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.14720"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyg.2020.01825"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.7860\/JCDR\/2014\/10258.5292"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.4088\/JCP.13r08908"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2010.12.017"},{"key":"e_1_2_11_28_2","doi-asserted-by":"publisher","DOI":"10.2174\/157488911795933901"},{"key":"e_1_2_11_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2017.08.049"},{"key":"e_1_2_11_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2019.03.016"},{"key":"e_1_2_11_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b11414"},{"key":"e_1_2_11_32_2","doi-asserted-by":"publisher","DOI":"10.31887\/DCNS.2016.18.4\/ncutler"},{"key":"e_1_2_11_33_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/9407962"},{"key":"e_1_2_11_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2020.e05412"},{"key":"e_1_2_11_35_2","first-page":"1","article-title":"Phytochemical Screening and Antidiarrheal Evaluation of Acetone Extract of Acacia sieberiana Var Woodii (Fabaceae) Stem Bark in Wistar Rats","volume":"3","author":"Zeuko\u00b4o M. E.","year":"2015","journal-title":"Academia Journal of Pharmacy and Pharmacology"},{"key":"e_1_2_11_36_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.959661"},{"key":"e_1_2_11_37_2","first-page":"799","article-title":"Antidiarrheal Evaluation of Aqueous and Ethanolic Leaf Extracts of Acacia sieberiana DC. (Fabaceae) in Albino Rats","volume":"3","author":"Dawurung C. J.","year":"2012","journal-title":"Asian Journal of Experimental Biological Sciences"},{"key":"e_1_2_11_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2005.08.049"},{"key":"e_1_2_11_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1203689109"},{"key":"e_1_2_11_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3597"},{"key":"e_1_2_11_41_2","first-page":"1499","article-title":"Tryptophan Lessens Reserpine Induced Anxiety, Depression and Memory Impairment by Modulating Oxidative Stress and Serotonergic Activity","volume":"34","author":"Samad N.","year":"2021","journal-title":"Pakistan Journal of Pharmaceutical Sciences"},{"key":"e_1_2_11_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2009.05.024"},{"key":"e_1_2_11_43_2","article-title":"General Methodological Principles for Research and Evaluation in Traditional Medicine WHO\/EDM\/TRM\/2000.1. Annex II","volume":"87","author":"WHO","year":"2000","journal-title":"Research Guidelines for the Evaluation of the Safety and Efficacy of Herbal Medicines, Manila, WHO Regional Office for the Western Pacific"},{"key":"e_1_2_11_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(94)90144-9"},{"key":"e_1_2_11_45_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039765"},{"key":"e_1_2_11_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-020-00539-x"},{"key":"e_1_2_11_47_2","doi-asserted-by":"publisher","DOI":"10.3791\/3769"},{"key":"e_1_2_11_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/266730a0"},{"key":"e_1_2_11_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2015.07.040"},{"key":"e_1_2_11_50_2","doi-asserted-by":"publisher","DOI":"10.4274\/tjps.galenos.2019.25902"},{"key":"e_1_2_11_51_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7257448"},{"key":"e_1_2_11_52_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/5946291"},{"key":"e_1_2_11_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(72)90132-7"},{"key":"e_1_2_11_54_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-017-0376-6"},{"key":"e_1_2_11_55_2","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/2656249"},{"key":"e_1_2_11_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(61)90145-9"},{"key":"e_1_2_11_57_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-017-4697-3"},{"key":"e_1_2_11_58_2","doi-asserted-by":"publisher","DOI":"10.1515\/intox-2017-0006"},{"key":"e_1_2_11_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2018.07.007"},{"key":"e_1_2_11_60_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12888-022-04065-0"},{"key":"e_1_2_11_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2021.04.017"},{"key":"e_1_2_11_62_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.j.pain.0000460334.49525.5e"},{"key":"e_1_2_11_63_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007383"},{"key":"e_1_2_11_64_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-014-1532-0"},{"key":"e_1_2_11_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2008.05.043"},{"key":"e_1_2_11_66_2","doi-asserted-by":"crossref","unstructured":"FreitasA. E. BudniJ. Lobat oK. R.et al. Antidepressant-Like Action of the Ethanolic Extract of Tabebuia Avellanedae in Mice: Evidence for the Involvement of the Monoaminergic System 2010.","DOI":"10.1016\/j.pnpbp.2009.12.010"},{"key":"e_1_2_11_67_2","doi-asserted-by":"publisher","DOI":"10.1101\/2022.03.16.484557"},{"key":"e_1_2_11_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2017.11.069"},{"key":"e_1_2_11_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpsychires.2014.03.002"},{"key":"e_1_2_11_70_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-022-01661-0"},{"key":"e_1_2_11_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2004.05.016"},{"key":"e_1_2_11_72_2","doi-asserted-by":"publisher","DOI":"10.2165\/00023210-200317010-00004"},{"key":"e_1_2_11_73_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2021.10.004"},{"key":"e_1_2_11_74_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2007.01.033"},{"key":"e_1_2_11_75_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2016.10.021"},{"key":"e_1_2_11_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2020.112720"},{"key":"e_1_2_11_77_2","doi-asserted-by":"publisher","DOI":"10.1080\/01616412.2016.1251711"},{"key":"e_1_2_11_78_2","doi-asserted-by":"publisher","DOI":"10.1097\/AIA.0b013e318034194e"},{"key":"e_1_2_11_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/b978-0-12-398314-5.00001-5"},{"key":"e_1_2_11_80_2","doi-asserted-by":"publisher","DOI":"10.4196\/kjpp.2014.18.2.183"},{"key":"e_1_2_11_81_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7901908"},{"key":"e_1_2_11_82_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-53630-3.00009-9"},{"key":"e_1_2_11_83_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-020-0310-6"},{"key":"e_1_2_11_84_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-021-02560-1"},{"key":"e_1_2_11_85_2","doi-asserted-by":"publisher","DOI":"10.1093\/fampra\/18.3.292"},{"key":"e_1_2_11_86_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24031866"},{"key":"e_1_2_11_87_2","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqab109"},{"key":"e_1_2_11_88_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2015.00037"},{"key":"e_1_2_11_89_2","doi-asserted-by":"publisher","DOI":"10.1177\/1534582305277152"},{"key":"e_1_2_11_90_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psyneuen.2007.10.006"},{"key":"e_1_2_11_91_2","doi-asserted-by":"publisher","DOI":"10.1002\/syn.20309"},{"key":"e_1_2_11_92_2","first-page":"93","article-title":"Determination of Substance Acute Toxicity by LD50","volume":"10","author":"Hodge H. C.","year":"1943","journal-title":"Am Ind Hyg Assoc"},{"key":"e_1_2_11_93_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2006.12.027"},{"key":"e_1_2_11_94_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2008.06.014"},{"key":"e_1_2_11_95_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2023.137441"},{"key":"e_1_2_11_96_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2012.01331.x"},{"key":"e_1_2_11_97_2","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12061353"},{"key":"e_1_2_11_98_2","doi-asserted-by":"publisher","DOI":"10.1166\/mex.2020.1851"},{"key":"e_1_2_11_99_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/3510423"},{"key":"e_1_2_11_100_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crphar.2023.100161"}],"container-title":["Advances in Pharmacological and Pharmaceutical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/adpp\/1244498","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/adpp\/1244498","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/adpp\/1244498","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T21:23:12Z","timestamp":1773004992000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/adpp\/1244498"}},"subtitle":[],"editor":[{"given":"Jirapornchai","family":"Suksaeree","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":100,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1155\/adpp\/1244498"],"URL":"https:\/\/doi.org\/10.1155\/adpp\/1244498","archive":["Portico"],"relation":{},"ISSN":["2633-4682","2633-4690"],"issn-type":[{"value":"2633-4682","type":"print"},{"value":"2633-4690","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1244498"}}