{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T16:26:49Z","timestamp":1778776009348,"version":"3.51.4"},"reference-count":68,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"vor","delay-in-days":133,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advances in Pharmacological and Pharmaceutical Sciences"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Desmos chinensis<\/jats:italic>\n                      (Annonaceae) is an underexplored medicinal plant from Southeast Asia with limited pharmacological validation. This study investigated, for the first time, the analgesic, anti\u2010pyretic, anti\u2010inflammatory, and anti\u2010arthritic potential of the methanolic extract of\n                      <jats:italic>D. chinensis<\/jats:italic>\n                      leaf and its solvent\u2010soluble fractions (n\u2010hexane, dichloromethane, and ethyl acetate) using in vivo, in vitro, and in silico approaches.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Analgesic activity was assessed using the acetic acid\u2013induced writhing method; anti\u2010pyretic activity was assessed using the brewer\u2019s yeast\u2010induced pyrexia model; anti\u2010inflammatory activity was assessed using protein denaturation; and anti\u2010arthritic activity was assessed using the human red blood cell membrane stabilization method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      In the writhing test, the ME and ethyl acetate fractions at 400\u2009mg\/kg produced marked analgesic effects, with 86.36% and 65.91% inhibition of writhing, respectively, compared with diclofenac (90.45%). In the anti\u2010pyretic model, ME significantly reduced yeast\u2010induced hyperthermia, achieving up to 100% reduction in pyrexia within 4\u2009h at 400\u2009mg\/kg (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001). In vitro assays showed concentration\u2010dependent anti\u2010inflammatory and anti\u2010arthritic activities. ME inhibited protein denaturation with an IC\n                      <jats:sub>50<\/jats:sub>\n                      of 337.42\u2009\u03bcg\/mL, while HRBC membrane stabilization revealed strong anti\u2010arthritic potential with an IC\n                      <jats:sub>50<\/jats:sub>\n                      of 246.34\u2009\u03bcg\/mL, comparable to diclofenac sodium. Docking analysis revealed favorable interactions between phytoconstituents and inflammation\u2010related targets.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study provides the first integrated pharmacological evidence that\n                      <jats:italic>D. chinensis<\/jats:italic>\n                      leaves possess significant analgesic, anti\u2010pyretic, anti\u2010inflammatory, and anti\u2010arthritic activities. The quantitative efficacy and in silico findings highlight\n                      <jats:italic>D. chinensis<\/jats:italic>\n                      as a promising source of bioactive compounds, warranting further isolation, mechanistic, and preclinical investigations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/adpp\/3277644","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T15:51:15Z","timestamp":1778773875000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Anti\u2010Nociceptive, Anti\u2010Inflammatory, Anti\u2010Pyretic, and Anti\u2010Arthritic Activity of Methanolic Extract of\n                    <i>Desmos chinensis<\/i>\n                    and Its Different Solvent Fractions: In Vivo, In Vitro, and In Silico Intervention"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5746-826X","authenticated-orcid":false,"given":"Nigar Sultana","family":"Prithy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0333-3008","authenticated-orcid":false,"given":"Ainun","family":"Nahar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1926-6407","authenticated-orcid":false,"given":"Md. 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