{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:13:04Z","timestamp":1790356384529,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T00:00:00Z","timestamp":1737072000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advances in Pharmacological and Pharmaceutical Sciences"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    A simple and efficient validated assay for quantifying 21\u2010deoxycortisol (21\u2010DOC), 17\u2010hydroxyprogesterone (17\u2010OHP), cortisol, and cortisone in human plasma has been developed using ultra\u2010high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC\u2010MS\/MS). Analysis of plasma samples were performed on Atlantis dC18 (3\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    m) column using a mobile phase of 20.0\u2009mM ammonium acetate and acetonitrile (50:50,\n                    <jats:italic>v<\/jats:italic>\n                    :\n                    <jats:italic>v<\/jats:italic>\n                    ) that was delivered at isocratic flow rate 0.3\u2009mL\/minute. After addition of d4\u2010cortisol as an internal standard (IS), plasma samples containing 21\u2010DOC, 17\u2010OHP, cortisol, and cortisone were extracted with mixture of dichloromethane and tert\u2010butylmethyl ether 1:2 (\n                    <jats:italic>v<\/jats:italic>\n                    \/\n                    <jats:italic>v<\/jats:italic>\n                    ). Analytes were detected and quantified in the positive ion mode of electrospray ionization using multiple reaction monitoring transition set at mass to charge (m\/z): 347.17\u2009\u27f6\u2009311.12, 331.17\u2009\u27f6\u200996.97, 363.11\u2009\u27f6\u2009121.00, 361.18\u2009\u27f6\u2009163.11, and 367.19\u2009\u27f6\u2009121.24 for 21\u2010DOC and 17\u2010OHP, cortisol, cortisone, and cortisol\u2010d4 (IS), respectively. The relationship between concentration and peak area response (analyte\/IS) were linear over the range of 0.25\u201350, 0.5\u2013100, 1\u2013200, and 2\u2013400\u2009ng\/mL for 21\u2010DOC, 17\u2010OHP, cortisone, and cortisol, respectively. The mean extraction recovery of the analytes was in the range of 83%\u201396%. The coefficient of variation within and between days was less than 13.6%, and the bias ranged from \u22129.2% to 12%. The measured level of cortisol, cortisone, and 17\u2010OHP was in the range of 21.9\u2013110, 4.33\u201312.71, and 0.37\u20131.4\u2009ng\/mL, respectively. Furthermore, the measured value of cortisone\u2013cortisol ratio was in the range of 0.08\u20130.21.\n                  <\/jats:p>","DOI":"10.1155\/adpp\/3859670","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T06:53:40Z","timestamp":1737096820000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Method Development and Clinical Utility for Simultaneous Measurement of 21\u2010Deoxycortisol, 17\u2010Hydroxyprogesterone, Cortisol, and Cortisone Levels in Human Plasma Using UHPLC\u2010MS\/MS"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5679-1820","authenticated-orcid":false,"given":"Syed N.","family":"Alvi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2401-6877","authenticated-orcid":false,"given":"Saleh 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