{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T20:30:34Z","timestamp":1767558634960,"version":"3.41.2"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T00:00:00Z","timestamp":1734652800000},"content-version":"vor","delay-in-days":354,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021726","name":"Direktorat Riset and Pengembangan, Universitas Indonesia","doi-asserted-by":"publisher","award":["NKB-598\/UN2.RST\/HKP.05.00\/2023"],"award-info":[{"award-number":["NKB-598\/UN2.RST\/HKP.05.00\/2023"]}],"id":[{"id":"10.13039\/501100021726","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advances in Pharmacological and Pharmaceutical Sciences"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Antithrombotic medications, including antiplatelet agents, are standard treatments for patients with hyperlipidemia who have a high risk of developing cardiovascular disease (CVD). The ongoing exploration of new antiplatelet agents with minimal bleeding effects is crucial, including the investigation of potential compounds derived from natural products. This study intended to evaluate the antiplatelet effects of a combined extract of sappan wood (<jats:italic>Caesalpinia sappan<\/jats:italic> L.) and red ginger (<jats:italic>Zingiber officinale<\/jats:italic> var. <jats:italic>Rubrum<\/jats:italic>) in high\u2010fat diet\u2010(HFD)\u2010induced rats.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Eighteen male Wistar rats were grouped into six groups (<jats:italic>n<\/jats:italic>\u2009=\u20093): control, negative, positive, and three groups of various combinations of extracts. All groups, excluding the control group, were fed an HFD for 8\u2009weeks. In the eighth week, the control and negative groups were given carboxyl methyl cellulose (CMC) 0.5%, the positive control group was administered aspirin, and the other three groups were administered the combination extract of sappan wood and red ginger at various doses for 2\u2009weeks. Blood samples were collected to assess the levels of hyperlipidemia and platelet hyperactivity markers by enzyme\u2010linked immunosorbent assay (ELISA). The physiological effects of platelet hyperactivity were evaluated using the tail bleeding assay.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> HFD\u2010induced hypertriglyceridemia and hypercholesterolemia synergistically enhanced platelet hyperactivity after 8\u2009weeks of induction. Interestingly, administration of all doses of the combined extract for 2\u2009weeks significantly decreased the platelet activation markers P\u2010selectin, RANTES, and PCSK9 in a dose\u2010dependent manner compared with the negative control. In addition, the combination of sappan wood and red ginger extract at dose 3 (sappan wood:red ginger: 200:800\u2009mg\/200 bw\/day) significantly extended the bleeding time of rats (<jats:italic>p<\/jats:italic> &lt; 0.05) compared to the negative control.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Collectively, our results highlight the antiplatelet effect of a combination of sappan wood and red ginger extract in HFD\u2010fed rats.<\/jats:p>","DOI":"10.1155\/adpp\/5543717","type":"journal-article","created":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T09:05:32Z","timestamp":1734685532000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antiplatelet Effects of a Combination of Sappan Wood (<i>Caesalpinia sappan<\/i> L.) and Red Ginger (<i>Zingiber officinale var. Rubrum<\/i>) Extracts in a High\u2010Fat Diet\u2010Induced Rat Model"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1550-8627","authenticated-orcid":false,"given":"Meidi Utami","family":"Puteri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nur","family":"Afifah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anisa Qisti","family":"Mathriul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhan Mahmudi","family":"Wicaksono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mellynia 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