{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T22:49:01Z","timestamp":1788043741697,"version":"build-2784847793"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":81,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Cellular Pathology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Not enough is known about how ophiopogonin D (OP\u2010D) works and the molecular mechanisms involved in nonalcoholic steatohepatitis (NASH). This study aimed to investigate the antifibrosis effect and underlying mechanism of OP\u2010D in NAFLD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The rats were fed a high\u2010fat diet (HFD) to simulate NAFLD. After OP\u2010D treatment or not, the rat serum lipid levels and inflammatory and ferroptosis\u2010related factors were detected. Online databases and network pharmacology were used to collect the targets of OP\u2010D against NASH. The effect of OP\u2010D on AKT1, STAT3, ferroptotic markers, and fibrotic markers was determined using western blot or PCR assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>OP\u2010D inhibited abnormal liver function, fibrotic markers (\u03b1\u2010SMA\/Col1\u03b11), and ferroptosis\u2010related factor (GPX4) in NASH rats. Network pharmacology proposed AKT1 and STAT3 as targets for OP\u2010D against NASH. OP\u2010D reduced the p\u2010AKT and p\u2010STAT3 in liver tissues. OP\u2010D reduced the levels of AKT1, STAT3, and HIF\u20101\u03b1 in model hepatic stellate cells. AKT1 and STAT3 overexpression reversed the antifibrosis and ferroptosis\u2010induced effects of OP\u2010D in model hepatic stellate cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>OP\u2010D may alleviate fibrosis in NASH by regulating ferroptosis via the AKT1\/STAT3\/HIF\u20101\u03b1 axis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/ancp\/5567109","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T05:42:09Z","timestamp":1774330929000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ophiopogonin D Alleviates Nonalcoholic Steatohepatitis via Ferroptosis Through AKT1\/STAT3\/HIF\u20101\u03b1 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