{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T21:45:47Z","timestamp":1778708747374,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,4]],"date-time":"2025-01-04T00:00:00Z","timestamp":1735948800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Cellular Pathology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Circular RNAs (circRNAs), covalently closed single\u2010stranded RNAs, have been implicated in cancer progression. A previous investigation revealed that circ\u2010ZEB1 is expressed abnormally in liver cancer. However, the roles of circ\u2010ZEB1 in non\u2010small cell lung cancer (NSCLC) are unknown.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    In this study, we used fluorescence in situ hybridization (FISH) and RT\u2010qPCR to study circ\u2010ZEB1 expression in NSCLC cells and tissues. A luciferase reporter assay was performed to validate downstream targets of circ\u2010ZEB1. Transwell migration, 5\u2010ethynyl\u201020\u2010deoxyuridine (EdU), and cell counting kit\u20108 (CCK8) assays were performed to assess proliferation and migration. In vivo metastasis and tumorigenesis assays were also performed to investigate circ\u2010ZEB1 functions during NSCLC.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Our results showed that circ\u2010ZEB1 expression was increased in NSCLC tissues and cells. circ\u2010ZEB1 downregulation suppressed NSCLC cell proliferation as well as migration in vitro and in vivo. Luciferase data confirmed EIF5A and miR\u2010491\u20105p as downstream targets of circ\u2010ZEB1. EIF5A overexpression and miR\u2010491\u20105p suppression reversed NSCLC cell migration post circ\u2010ZEB1 silencing.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    Our collective findings advised that circ\u2010ZEB1 takes part in the malignant progression through regulating the miR\u2010491\u20105p\/EIF5A axis, highlighting its potential as an effective NSCLC therapeutic target.\n                  <\/jats:p>","DOI":"10.1155\/ancp\/5595692","type":"journal-article","created":{"date-parts":[[2025,1,4]],"date-time":"2025-01-04T08:24:48Z","timestamp":1735979088000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["circ\u2010ZEB1 Enhances NSCLC Metastasis and Proliferation by Modulating the miR\u2010491\u20105p\/EIF5A 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