{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T11:39:16Z","timestamp":1791545956298,"version":"4.3.4"},"reference-count":99,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"vor","delay-in-days":140,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aquaculture Nutrition"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    The microalgae\n                    <jats:italic>Nannochloropsis oceanica<\/jats:italic>\n                    cultivated from brewery waste was considered a sustainable feed ingredient for gilthead sea bream (\n                    <jats:italic>Sparus aurata<\/jats:italic>\n                    ), evaluating physiological, molecular and microbial responses. Over 96 days, fish were fed four isoproteic and isolipidic diets: a commercial\u2010based diet (D1), and three fishmeal\u2010free diets with increasing\n                    <jats:italic>Nannochloropsis<\/jats:italic>\n                    inclusion levels (0%, D2; 2%, D3; 14%, D4). The D4 diet was additionally supplemented with docosahexaenoic acid (DHA)\u2010rich algae oil instead of fish oil (FO). Growth performance remained almost equal in fish fed all the fishmeal\u2010free diets with only a slight and final improvement (&lt;4%) in the control D1 fish, although an enhanced expression with a discriminant capacity was reported for hepatic growth\u2010related markers (\n                    <jats:italic>igf1<\/jats:italic>\n                    and\n                    <jats:italic>ghr1<\/jats:italic>\n                    ) in D4 fish. Gene expression profiling also revealed a down\u2010regulation of fatty acid synthesis in combination with the up\u2010regulation of tissue lipid\u2010uptake genes, mostly linked to the dietary DHA enrichment. Antioxidant capacity was increased in both D3\u2013D4 fish, suggesting a progressive activation of enzymatic and non\u2010enzymatic defences with the dietary microalgae diets (D3\u2013D4). Moreover, intestinal transcriptomic profiling revealed a tightly regulated immune milieu, with coordinated modulation of pro\u2010 and anti\u2010inflammatory pathways indicative of an immune surveillance state induced by dietary microalgae. Water microbiota also shifted with diet composition, indicating a link between feeding strategy and environmental microbial communities. Taking all together,\n                    <jats:italic>Nannochloropsis<\/jats:italic>\n                    inclusion, particularly when combined with DHA\u2010rich microalgae oil, enhances fish health, immune and antioxidant status and beneficial gut microbiota markers. Altogether makes the circularly\u2010produced\n                    <jats:italic>Nannochloropsis oceanica<\/jats:italic>\n                    a valuable resource for the sustainable development of aquaculture feeds.\n                  <\/jats:p>","DOI":"10.1155\/anu\/1003936","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T07:41:58Z","timestamp":1779349318000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["From Brewery Waste to Sustainable Aquafeed: Harnessing\n                    <i>Nannochloropsis<\/i>\n                    Microalgae for Fishmeal\u2010Free Gilthead Sea Bream Diets"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0553-109X","authenticated-orcid":false,"given":"Fernando","family":"Naya-Catal\u00e0","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2933-236X","authenticated-orcid":false,"given":"Ricardo","family":"Domingo-Bret\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7349-1718","authenticated-orcid":false,"given":"Qinge","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0933-8895","authenticated-orcid":false,"given":"Paul George","family":"Holhorea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9186-3463","authenticated-orcid":false,"given":"\u00c1lvaro","family":"Belenguer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1566-5956","authenticated-orcid":false,"given":"Federico","family":"Moroni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3124-5986","authenticated-orcid":false,"given":"Josep","family":"Calduch-Giner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1804-8083","authenticated-orcid":false,"given":"Alla","family":"Silkina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danny","family":"Van Mullem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7728-8888","authenticated-orcid":false,"given":"Ronald","family":"Halim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2506-1523","authenticated-orcid":false,"given":"Jaume","family":"P\u00e9rez-S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,21]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ocecoaman.2022.106269"},{"key":"e_1_2_11_2_2","volume-title":"Microbial Electrochemical and Fuel Cells","author":"Gude V. 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