{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:03:37Z","timestamp":1790147017509,"version":"4.0.1"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":264,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32202947"],"award-info":[{"award-number":["32202947"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aquaculture Nutrition"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Metabolic disorders induced by high\u2010starch diets are the primary cause limiting the use of carbohydrates in aquafeeds. This study aimed to investigate the intervention effects of zinc on metabolic disorders induced by high\u2010starch diets in\n                    <jats:italic>Micropterus salmoides<\/jats:italic>\n                    . Five diets were formulated, including a control diet (115\u2009g\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    starch) and three high\u2010starch diets (163\u2009g\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    starch) supplemented with 0 (HS\u2010LZn), 20 (HS\u2010MZn) and 150 (HS\u2010HZn) mg\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    Zn. After 8 weeks of feeding, intraperitoneal glucose (Glu) tolerance tests (IPGTTs) and intraperitoneal insulin tolerance tests (IPITTs) were conducted to evaluate insulin sensitivity. High\u2010starch diets inhibited feed intake but improved feed utilization. Diet HS\u2010HZn significantly increased Zn content in the hepatopancreas. Zn retention decreased significantly as the dietary Zn supplementation level increased from 0 to 150\u2009mg\/kg. High\u2010starch diets significantly improved the retentions of protein and energy. High\u2010starch diets exerted more pronounced effects on glycogen metabolism and glycolysis. Diets HS\u2010LZn and HS\u2010MZn induced severe hepatic glycogen accumulation, concomitant with significant decreases in activities of hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), glycogen synthase (GS) kinase\u20103\u03b2 (GSK\u20103\u03b2), and acid alpha\u2010glucosidase (GAA). Dietary 150\u2009mg\/kg Zn supplementation significantly decreased hepatic glycogen and increased activities of HK, PFK, citrate synthase (CS), and GAA. High\u2010starch diets did not induce severe lipid metabolic disturbances or obvious fat deposition. The insulin tolerance test revealed that the area under the curve (AUC) and incremental AUC (iAUC) for Glu in the HS\u2010LZn group were significantly higher than the control and HS\u2010HZn groups. Meanwhile, phosphorylated AKT (p\u2010AKT) level and immunofluorescence intensity were significantly decreased in the HS\u2010LZn group, while zinc improved AKT phosphorylation. Untargeted metabolomics analysis demonstrated that high\u2010starch diets without zinc supplementation inhibited multiple amino acid metabolic pathways.\n                  <\/jats:p>","DOI":"10.1155\/anu\/2761195","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:34:35Z","timestamp":1790145275000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nutritional Intervention of Zinc on Metabolic Disorders Induced by High\u2010Starch Diets in Largemouth Bass (\n                    <i>Micropterus salmoides<\/i>\n                    ): Insights From Glycogen Metabolism and Insulin Sensitivity"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4410-4381","authenticated-orcid":false,"given":"Bo","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5373-473X","authenticated-orcid":false,"given":"Xiaoxuan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2117-8755","authenticated-orcid":false,"given":"Ziyu","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9278-190X","authenticated-orcid":false,"given":"Bo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0854-529X","authenticated-orcid":false,"given":"Jiaming","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6837-2182","authenticated-orcid":false,"given":"Baoping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1442-7809","authenticated-orcid":false,"given":"Zhiyong","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1147-0190","authenticated-orcid":false,"given":"Xiaofang","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0625-3918","authenticated-orcid":false,"given":"Wanjie","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0503-7705","authenticated-orcid":false,"given":"Yuexing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/raq.70022"},{"key":"e_1_2_13_2_2","first-page":"207","volume-title":"Recent Advances in Animal Nutrition and Metabolism,\u201d","author":"Li X.","year":"2021"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/5134049"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2011.06.014"},{"key":"e_1_2_13_5_2","first-page":"19","article-title":"Insulin and Insulin Resistance","volume":"26","author":"Wilcox G.","year":"2005","journal-title":"The Clinical Biochemist Reviews"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1615\/JEnvironPatholToxicolOncol.v25.i3.40"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.3746\/pnf.2017.22.1.1"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-018-1308-z"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.mpg.0000166800.54085.9c"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2008.045856"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.3945\/jn.109.119677"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1139\/apnm-2018-0519"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2023.127375"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu16121819"},{"key":"e_1_2_13_15_2","unstructured":"BehnkeK. 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