{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T12:54:22Z","timestamp":1791550462119,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":281,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aquaculture Nutrition"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    The American cockroach (\n                    <jats:italic>Periplaneta americana<\/jats:italic>\n                    ) is an emerging sustainable source of insect\u2010based chitin, yet its physiological effects and optimal inclusion levels in herbivorous fish diets remain undefined. This study evaluated the dose\u2010dependent effects of dietary\n                    <jats:italic>P. americana<\/jats:italic>\n                    chitin (0%, 2.5%, 5%, 10%, and 20%) on juvenile grass carp (\n                    <jats:italic>Ctenopharyngodon idella<\/jats:italic>\n                    ) over a 63\u2010day trial. Results revealed a distinct biphasic response in growth and nutrient utilization. Fish fed the 5% chitin diet exhibited the highest weight gain and specific growth rate (SGR), coinciding with the highest apparent digestibility coefficient (ADC) of crude protein (74.34%) compared with the control (72.01%). Conversely, higher inclusion levels (10% and 20%) significantly suppressed growth and reduced protein digestibility. Metagenomic reanalysis showed that the riboflavin\u2010biosynthesis gene ribA (K01497) was significantly reduced at 2.5%\u201310% dietary chitin (F (4,10)\u2009=\u20097.78, FDR\n                    <jats:italic>p<\/jats:italic>\n                    = 0.041), while the overall riboflavin metabolism pathway, the M00125 biosynthesis module, and intestinal riboflavin levels did not differ significantly among diets. Among the inclusion levels tested, 5% produced the most favorable overall response under the present experimental conditions, balancing improved growth and protein digestibility with the negative responses observed at higher inclusion levels.\n                  <\/jats:p>","DOI":"10.1155\/anu\/3097646","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T12:28:23Z","timestamp":1791548903000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary American Cockroach (\n                    <i>Periplaneta americana<\/i>\n                    ) Chitin Influences Growth Performance, Nutrient Utilization, Gut Microbiota, and Riboflavin\u2010Related Metabolism in Juvenile Grass Carp (\n                    <i>Ctenopharyngodon idella<\/i>\n                    )"],"prefix":"10.1155","volume":"2026","author":[{"given":"Xiaofang","family":"Zou","sequence":"first","affiliation":[{"name":"College of Agriculture and Biological Science Dali University  Dali Yunnan China  dali.edu.cn"},{"name":"Cangshan Forest Ecosystem Observation and Research Station of Yunnan Province  Dali Yunnan China"},{"name":"Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province Dali University  Dali Yunnan China  dali.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiujie","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biological Science Dali University  Dali Yunnan China  dali.edu.cn"},{"name":"Cangshan Forest Ecosystem Observation and Research Station of Yunnan Province  Dali Yunnan China"},{"name":"Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province Dali University  Dali Yunnan China  dali.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Pu","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biological Science Dali University  Dali Yunnan China  dali.edu.cn"},{"name":"Cangshan Forest Ecosystem Observation and Research Station of Yunnan Province  Dali Yunnan China"},{"name":"Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province Dali University  Dali Yunnan China  dali.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biological Science Dali University  Dali Yunnan China  dali.edu.cn"},{"name":"Cangshan Forest Ecosystem Observation and Research Station of Yunnan Province  Dali Yunnan China"},{"name":"Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province Dali University  Dali Yunnan China  dali.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongshou","family":"Yang","sequence":"additional","affiliation":[{"name":"National-Local Joint Engineering Research Center of Entomoceutics Dali University  Dali Yunnan China  dali.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0445-8196","authenticated-orcid":false,"given":"Xiaowen","family":"Long","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biological Science Dali University  Dali Yunnan China  dali.edu.cn"},{"name":"Cangshan Forest Ecosystem Observation and Research Station of Yunnan Province  Dali Yunnan China"},{"name":"Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province Dali University  Dali Yunnan China  dali.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-819148-4.00008-7"},{"key":"e_1_2_12_2_2","first-page":"1","article-title":"Distribution of Digestive Enzymes in the Gut of American Cockroach, (Periplaneta americana)","volume":"4","author":"Oyebanji O.","year":"2014","journal-title":"International Journal of Scientific and Research Publications"},{"key":"e_1_2_12_3_2","first-page":"240","article-title":"Advances in Research on Active Ingredients and Related Pharmacology of Periplaneta americana","volume":"27","author":"Gao Y. 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Dietary American Cockroach (Periplaneta americana) Chitin Modulates Growth Nutrient Digestibility and the Gut Microbiota-Riboflavin Metabolic Axis in Juvenile Grass Carp (Ctenopharyngodon idella) 2025 Elsevier SSRN Electronic Journalhttps:\/\/papers.ssrn.com\/sol3\/papers.cfm?abstract_id=5388360."}],"container-title":["Aquaculture Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/anu\/3097646","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/anu\/3097646","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/anu\/3097646","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T12:28:26Z","timestamp":1791548906000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/anu\/3097646"}},"subtitle":[],"editor":[{"given":"Linghong","family":"Miao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/anu\/3097646"],"URL":"https:\/\/doi.org\/10.1155\/anu\/3097646","archive":["Portico"],"relation":{},"ISSN":["1353-5773","1365-2095"],"issn-type":[{"value":"1353-5773","type":"print"},{"value":"1365-2095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-01-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3097646"}}