{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:26:21Z","timestamp":1787243181342,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":231,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aquaculture Nutrition"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Optimal feeding strategies for the red claw crayfish,\n                    <jats:italic>Cherax quadricarinatus<\/jats:italic>\n                    , remain to be established. In this study,\n                    <jats:italic>C. quadricarinatus<\/jats:italic>\n                    was fed frozen housefly larva meal (\n                    <jats:italic>Musca domestica<\/jats:italic>\n                    [MD]) at different frequencies to evaluate effects on growth performance, antioxidant capacity, digestive enzyme activity, immune genes, and intestinal microbiota structure. Four feeding protocols were established: control (C) fed basic feed, experimental group 1 (E1) fed basic feed for 2 days and then compound feed (50% MD\u2009+\u200950% basic feed) for 1 day, experimental group 2 (E2) fed basic feed for 1 day and then compound feed for 1 day, and experimental group 3 (E3) fed compound feed daily. After 10 weeks, the weight gain rate (WGR) was significantly higher in E3 than in the other three groups (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05), while the survival rate (SR), specific growth rate (SGR), and hepatosomatic index (HSI) did not differ among groups (\n                    <jats:italic>p<\/jats:italic>\n                    &gt; 0.05). In terms of physiological processes, lipase (LPS) and trypsin (TPS) activities were higher, expression levels of the immune\u2010related gene lysozyme (\n                    <jats:italic>LZM<\/jats:italic>\n                    ) were significantly higher, and acid phosphatase (ACP) activity was significantly higher in E2 than in the control group (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). E2 showed the highest ecdysone receptor (\n                    <jats:italic>EcR<\/jats:italic>\n                    ) and retinoid X receptor (\n                    <jats:italic>RXR<\/jats:italic>\n                    ) expression levels, related to molting regulation. In the intestinal microbiota, there were no differences in \u03b1\u2010diversity indices among groups; however, E1 increased the abundance of the phylum Bacteroidota. Consequently, while daily MD feeding (E3) maximized weight gain, intermittent feeding (E2) offered a superior balance between growth performance and physiological homeostasis, including digestion, immunity, and molting regulation. These findings provide a theoretical basis for precision farming of\n                    <jats:italic>C. quadricarinatus<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1155\/anu\/9988505","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T10:14:27Z","timestamp":1787220867000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of the Feeding Frequency of Frozen Housefly Larvae on the Growth Performance, Physiological Functions, and Intestinal Microbiota of\n                    <i>Cherax quadricarinatus<\/i>"],"prefix":"10.1155","volume":"2026","author":[{"given":"Zhuang","family":"Mai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3967-2379","authenticated-orcid":false,"given":"Jie","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4704-6084","authenticated-orcid":false,"given":"Yakun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sikai","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoyan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianhui","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7959-6075","authenticated-orcid":false,"given":"Lingyun","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"e_1_2_11_1_2","volume-title":"The State of World Fisheries and Aquaculture 2024: Blue Transformation in Action","author":"FAO (Food and Agriculture Organization of the UN)","year":"2024"},{"key":"e_1_2_11_2_2","first-page":"217","article-title":"Effects of Iced Wild Fish and Formulated Diet on Growth Performance, Intestinal Structure and Microflora Diversity of Marbled Flounder (Pseudopleuronectes yokohamae)","volume":"38","author":"Xu X. 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