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Prominent examples include human respiratory syncytial virus (HRSV), Ebola virus (EBOV), rabies virus (RABV), and Marburg virus (MARV). In addition to their clinical severity, these viruses are considered potential biological threats due to their high transmissibility and virulence. In this study, we performed a metatranscriptomic analysis of\n                    <jats:italic>Culex<\/jats:italic>\n                    and\n                    <jats:italic>Anopheles<\/jats:italic>\n                    mosquitoes collected at the DBB (Diretoria de Biodiversidade e Biotecnologia), S\u00e3o Paulo, Brazil. Our analysis revealed viral sequences associated with two families within\n                    <jats:italic>Mononegavirales<\/jats:italic>\n                    :\n                    <jats:italic>Lispiviridae<\/jats:italic>\n                    and\n                    <jats:italic>Rhabdoviridae<\/jats:italic>\n                    . Phylogenetic analysis of the\n                    <jats:italic>Lispiviridae<\/jats:italic>\n                    sequences identified six variants, designated\n                    <jats:italic>CxLispV-SP_03<\/jats:italic>\n                    ,\n                    <jats:italic>CxLispV-SP_09<\/jats:italic>\n                    ,\n                    <jats:italic>CxLispV-SP_12<\/jats:italic>\n                    ,\n                    <jats:italic>CxLispV-SP_13<\/jats:italic>\n                    ,\n                    <jats:italic>CxLispV-SP_14<\/jats:italic>\n                    , and\n                    <jats:italic>CxLispV-SP_15<\/jats:italic>\n                    , that formed a strongly supported monophyletic clade with Canya virus, suggesting the discovery of a putative novel viral lineage. Examination of RNA\u2010dependent RNA polymerase (RdRp) domains in these sequences confirmed the presence of essential catalytic motifs. In contrast, the detected rhabdoviruses exhibited greater genomic structural heterogeneity, including the presence of accessory protein domains. Pairwise comparisons based on RdRp amino acid identity delineated four distinct groups among these sequences, pointing to substantial evolutionary divergence within the sampled\n                    <jats:italic>rhabdovirus<\/jats:italic>\n                    diversity. Together, these findings contribute to the growing characterization of mosquito\u2010associated mononegaviruses and provide a genomic foundation to support future surveillance efforts and public health risk assessments related to vector\u2010borne viruses.\n                  <\/jats:p>","DOI":"10.1155\/av\/8104754","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T05:51:14Z","timestamp":1782798674000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Putative Novel Viruses in the Families\n                    <i>Lispiviridae<\/i>\n                    and\n                    <i>Rhabdoviridae<\/i>\n                    Detected in\n                    <i>Culex<\/i>\n                    and\n                    <i>Anopheles<\/i>\n                    Mosquitoes Collected at the S\u00e3o Paulo 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