{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T14:00:05Z","timestamp":1792504805881,"version":"4.5.0"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T00:00:00Z","timestamp":1781395200000},"content-version":"vor","delay-in-days":164,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Bioinorganic Chemistry and Applications"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    This study aimed to biosynthesize silver nanoparticles using\n                    <jats:italic>Elaeagnus angustifolia<\/jats:italic>\n                    flower\n                    <jats:italic>buds<\/jats:italic>\n                    \u2014an Iranian medicinal plant extract rich in bioactive phytochemicals and evaluate their antimicrobial potential through complementary broth microdilution (MIC\/MBC) and agar well diffusion assays, alongside an assessment of their anticancer and biomolecular interaction properties. The synthesis leverages natural reducing and capping agents (phenols, flavonoids, tannins) to produce stable, spherical AgNPs (FEA@AgNPs), characterized by UV\u2013vis (\n                    <jats:italic>\u03bb<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    \u2009=\u2009439\u2009nm), XRD (\u223c10\u2009nm, face\u2010centered cubic), FESEM, TEM (6.61\u2009nm), and FT\u2010IR. A zeta potential of \u221232.68\u2009mV confirmed colloidal stability. The nanoparticles were stable for over a month, indicating that\n                    <jats:italic>E. angustifolia<\/jats:italic>\n                    flower aqueous extract is suitable for their preparation and stabilization. FEA@AgNPs showed moderate activity against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    (12 vs. 24\u2009mm for gentamicin) and no agar diffusion inhibition against\n                    <jats:italic>E. coli<\/jats:italic>\n                    , despite a MIC of 37.5\u2009\u03bcg\/mL in the broth assay. The antioxidant results show 55% DPPH radical scavenging at 160\u2009\u03bcg\/mL. Notably, they induced dose\u2010 and time\u2010dependent cytotoxicity in PC3 and AGS cancer cells, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 7.49 and 5.33\u2009\u03bcg\/mL, respectively, after 72\u2009h (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). Spectroscopic analyses revealed a strong binding affinity to calf thymus DNA and human serum albumin, suggesting biomolecular interaction capacity relevant to drug delivery. This work provides a green, efficient route to multifunctional AgNPs, bridging traditional herbal knowledge and bioinorganic nanomedicine for potential applications in infection control and oncology.\n                  <\/jats:p>","DOI":"10.1155\/bca\/9779231","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T06:42:08Z","timestamp":1781505728000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Plant\u2010Mediated Fabrication of Silver Nanoparticles Using\n                    <i>Elaeagnus angustifolia<\/i>\n                    Flowers: In Vitro Evaluation of Antibacterial, Antifungal, Antioxidant, and Toxic Effects and Biomolecular Interactions With ct\u2010DNA and HSA"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2687-3231","authenticated-orcid":false,"given":"Nahid","family":"Shahabadi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8144-3998","authenticated-orcid":false,"given":"Kani","family":"Shalmashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6325-4964","authenticated-orcid":false,"given":"Sedigheh","family":"Shokraei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5007-5110","authenticated-orcid":false,"given":"Leila","family":"Soltani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,14]]},"reference":[{"key":"e_1_2_8_1_2","first-page":"231","article-title":"Phenolic and Flavonoid Content of Elaeagnus angustifolia L. 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