{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T06:14:55Z","timestamp":1778739295569,"version":"3.51.4"},"reference-count":83,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":132,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Eichhornia crassipes<\/jats:italic>\n                      (water hyacinth) is an invasive aquatic plant traditionally regarded as an environmental nuisance but increasingly recognized for its phytopharmacological potential.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study is aimed at isolating, characterizing, and evaluating the pharmacological activities of bioactive compounds from the dichloromethane (DCM) fraction of the methanolic extract of\n                      <jats:italic>E. crassipes<\/jats:italic>\n                      flowers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Compounds were isolated through chromatographic techniques and structurally elucidated using\n                      <jats:sup>1<\/jats:sup>\n                      H NMR. Pharmacological activities were assessed using the brine shrimp lethality bioassay for cytotoxicity, egg white\u2013induced edema and human red blood cell membrane stabilization for anti\u2010inflammatory effects, and oral glucose tolerance tests in mice for hypoglycemic activity. Molecular docking against NF\u2010\n                      <jats:italic>\u03ba<\/jats:italic>\n                      B, TNF\u2010\n                      <jats:italic>\u03b1<\/jats:italic>\n                      , EGFR, BCL\u20102, GLUT3, and\n                      <jats:italic>\u03b1<\/jats:italic>\n                      \u2010amylase, along with ADMET and radar plot analyses, was performed to predict interactions, pharmacokinetics, and drug\u2010likeness.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and Discussion<\/jats:title>\n                    <jats:p>\n                      Five compounds were isolated: 4\u2009\n                      <jats:sup>\u2032<\/jats:sup>\n                      ,5,7\u2010trihydroxyflavone or apigenin (Compound 1), 3\u2009\n                      <jats:sup>\u2032<\/jats:sup>\n                      ,4\u2009\n                      <jats:sup>\u2032<\/jats:sup>\n                      ,5,7\u2010tetrahydroxyflavone or luteolin (Compound 2), 5,6,7,8,4\u2009\n                      <jats:sup>\u2032<\/jats:sup>\n                      \u2010pentahydroxyflavone or nortangeretin (Compound 3), methyl 3,5\u2010dihydroxy\u20104\u2010methoxybenzoate (Compound 4), and 4\u2010hydroxybenzoic acid (Compound 5). Both the crude methanolic extract (CME) and the DCM\u2010soluble fraction (DSF) exhibited strong cytotoxic activity in the brine shrimp lethality assay (LC\u2085\u2080\u2009&lt; 2\u2009\n                      <jats:italic>\u03bc<\/jats:italic>\n                      g\/mL). The DSF further demonstrated potent membrane stabilization (72% protection at 100\u2009\n                      <jats:italic>\u03bc<\/jats:italic>\n                      g\/mL) and marked hypoglycemic effects. Also, the CME illustrates dose\u2010dependent anti\u2010inflammatory activity in the egg white\u2013induced paw edema model (up to 38.96% inhibition at 600\u2009mg\/kg) and showed notable hypoglycemic effects in the oral glucose tolerance test in mice. Docking revealed strong binding affinities of flavonoids with key inflammatory and metabolic targets, outperforming reference drugs in several cases. ADMET profiling indicated favorable oral bioavailability, whereas the BOILED\u2010Egg model confirmed efficient gastrointestinal absorption but limited blood\u2013brain barrier penetration.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This first report of pure compound isolation from\n                      <jats:italic>E. crassipes<\/jats:italic>\n                      flowers highlights their multitarget pharmacological potential. The findings suggest that\n                      <jats:italic>E. crassipes<\/jats:italic>\n                      , often seen as an ecological burden, represents a sustainable reservoir of bioactive molecules with promising drug discovery applications.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/bmri\/1209329","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T05:52:12Z","timestamp":1778737932000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First Time Isolation Report of Bioactive Flavonoids From Water Hyacinth (\n                    <i>Eichhornia crassipes<\/i>\n                    ) Flower Associated With Pharmacological Investigation"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5319-1398","authenticated-orcid":false,"given":"Hasin","family":"Hasnat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7393-0275","authenticated-orcid":false,"given":"Md. 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