{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T04:03:52Z","timestamp":1784865832033,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":34,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005872","name":"Bahir Dar University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005872","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007941","name":"Addis Ababa University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007941","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007051","name":"Uppsala Universitet","doi-asserted-by":"publisher","award":["ETH:02"],"award-info":[{"award-number":["ETH:02"]}],"id":[{"id":"10.13039\/501100007051","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Currently available drugs for trypanosomiasis are few, and their use is limited by toxicity and growing resistance. This highlights the need for safer and more effective alternatives. In this study, the in vitro and in vivo antitrypanosomal activities of an 80% methanol root extract of\n                    <jats:italic>Lobelia rhynchopetalum<\/jats:italic>\n                    and its major constituent were evaluated against\n                    <jats:italic>Trypanosoma congolense<\/jats:italic>\n                    field isolates. Phytochemical separation of the extract yielded lobetyolin (a polyacetylene compound), confirmed through spectroscopic analysis. At 4\u2009mg\/mL, both the crude extract and lobetyolin inhibited parasite motility, with lobetyolin acting slightly faster (25\u2009min) than the extract (30\u2009min). The blood incubation assay demonstrated dose\u2010dependent protection, with 4\u2009mg\/mL of DA, the lobetyolin\u2010rich extract, or pure lobetyolin preventing infection, while lower doses only delayed parasitemia. In vivo testing showed that lobetyolin at 100\u2009mg\/kg exhibited stronger activity than the crude extract at 400\u2009mg\/kg. Molecular docking demonstrated that lobetyolin binds strongly to the homology\u2010modeled trypanothione reductase (TR) enzyme of\n                    <jats:italic>T. congolense<\/jats:italic>\n                    , achieving a Glide score of \u22128.002\u2009kcal\/mol, which is close to that of the native ligand (\u22128.307\u2009kcal\/mol). This suggests that lobetyolin may inhibit TR, thereby disrupting the parasite\u2032s redox balance essential for survival. Overall, these findings provide the first evidence supporting the antitrypanosomal activity of\n                    <jats:italic>L. rhynchopetalum<\/jats:italic>\n                    root extract and lobetyolin, providing scientific support to the plant\u2032s traditional use. Further studies are needed to fully validate its therapeutic potential.\n                  <\/jats:p>","DOI":"10.1155\/bmri\/2214310","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T06:09:07Z","timestamp":1770271747000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antitrypanosomal Activity and Molecular Docking Studies of Lobetyolin From\n                    <i>Lobelia rhynchopetalum<\/i>\n                    Hemsl. Root Extract Against\n                    <i>Trypanosoma congolense<\/i>\n                    Field Isolates"],"prefix":"10.1155","volume":"2026","author":[{"given":"Selamawit","family":"Yimer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eyael","family":"Tewelde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4007-6443","authenticated-orcid":false,"given":"Daniel","family":"Bisrat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9966-2236","authenticated-orcid":false,"given":"Solomon","family":"Tadesse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0918-3635","authenticated-orcid":false,"given":"Mariamawit Y.","family":"Yeshak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"crossref","DOI":"10.1002\/9781683673903","volume-title":"Forgotten Diseases: The Neglected Tropical Diseases and Their Impact on Global Health and Development","author":"Hotez P. 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