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Aberrant SNCA expression exerts neurotoxicity in PD by disrupting mitochondrial function, promoting protein aggregation or oxidative stress, ultimately leading to neuronal cell death. Numerous studies have hypothesized that SNCA is a tumor suppressor gene, but the underlying mechanism remains elusive. In this study, the SNCA gene is delivered to NSCLC cells via transfection of a plasmid vector with carbonate apatite (CA) nanoparticles as the delivery vehicle. Biochemical assays including cytotoxicity assay, oxidative stress assay, flow cytometry, and caspase activity assay were performed to assess the effects of SNCA overexpression in NSCLC cells. SNCA\u2010overexpressed NSCLC cells were established using the optimized CA\/plasmid complexes. SNCA overexpression promoted oxidative stress\u2013induced cell death and apoptosis in both lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) cells. 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