{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:26:38Z","timestamp":1772987198398,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T00:00:00Z","timestamp":1753488000000},"content-version":"vor","delay-in-days":206,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007956","name":"Nanjing University of Chinese Medicine","doi-asserted-by":"publisher","award":["XZR2021082"],"award-info":[{"award-number":["XZR2021082"]}],"id":[{"id":"10.13039\/501100007956","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Objective:<\/jats:bold>\n                    The objective is to explore the potential pathogenesis and therapeutic mechanism of Yinchenhao decoction (YCHD) in intrahepatic cholestasis of pregnancy (ICP) by focusing on the regulatory role of exosomal miR\u2010370\u20103p on target genes TM9SF4 and KIT.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    Exosomes were isolated from the serum samples of normal pregnant women (control), patients with ICP, HTR\u20108\/SVneo cells, and Sprague\u2013Dawley (SD) pregnant rats via differential centrifugation. Characterization of these exosomes was performed using electron microscopy, nanoparticle tracking analysis (NTA), and western blotting. Quantitative reverse transcription PCR (qRT\u2010PCR) and the bioinformatics tool starBase were used to identify miR\u2010370\u20103p as a candidate miRNA. Dual\u2010luciferase reporter assays were used to confirm that TM9SF4 and KIT are direct targets of miR\u2010370\u20103p. An in vitro ICP cell model was established using HTR\u20108\/SVneo cells to investigate the interactions between miR\u2010370\u20103p and its targets. An animal model was established to validate the targeted regulation of miR\u2010370\u20103p on TM9SF4 and KIT, as well as the therapeutic effect of YCHD\n                    <jats:italic>in vivo<\/jats:italic>\n                    .\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    The exosomal miR\u2010370\u20103p expression was significantly upregulated, whereas the TM9SF4 and KIT expressions were downregulated as demonstrated by qRT\u2010PCR and western blot analyses. RNA pull\u2010down assays confirmed a direct negative regulatory relationship between miR\u2010370\u20103p and both TM9SF4 and KIT at the molecular level. Finally, the therapeutic potential of YCHD was verified by its ability to reverse the altered expression patterns of miR\u2010370\u20103p, TM9SF4, and KIT in the animal ICP model.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    Our study demonstrates that YCHD protects against ICP through the miR\u2010370\u20103p\/TM9SF4\/KIT axis, suggesting miR\u2010370\u20103p as a potential therapeutic target for ICP.\n                  <\/jats:p>","DOI":"10.1155\/bmri\/3000226","type":"journal-article","created":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T14:04:11Z","timestamp":1753538651000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Yinchenhao Decoction Protects Against Intrahepatic Cholestasis During Pregnancy Through the miR\u2010370\u20103p\/TM9SF4\/KIT 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