{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T14:03:15Z","timestamp":1791813795560,"version":"4.3.4"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":160,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Fisheries play a significant role in food security, livelihood, and source of income in developing countries. Despite this, fish can spread infections.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A cross\u2010sectional study with a simple random sampling approach was conducted to determine the prevalence and antibiotic susceptibility profile of\n                      <jats:italic>E. coli<\/jats:italic>\n                      or O157:H7 isolate from fish and fish handling equipment (knife) in Lake Ziway, Batu town, Ethiopia. To isolate and identify\n                      <jats:italic>E. coli<\/jats:italic>\n                      O157:H7, a total of 220 samples were collected by simple random sampling approach. Muscle and skin samples were collected from each 120 fish comprising Nile tilapia, common carp, and catfish obtained from landing sites and cold storage establishments of restaurants. One hundred samples were collected from fish processing knives and the hands of fish handlers. All the microbial identification and isolation procedures were conducted based on selective culture media isolations. Antimicrobial susceptibility was conducted based on Kirby\u2013Bauer disk diffusion protocols.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Findings<\/jats:title>\n                    <jats:p>\n                      Overall prevalence of O157:H7 was found (4.5%).\n                      <jats:italic>E. coli<\/jats:italic>\n                      O157:H7 was detected in two (3.3%) of the total 100 fish handling equipment and eight (6.7%) of the total 120 fish samples.\n                      <jats:italic>E. coli<\/jats:italic>\n                      O157:H7 was isolated at 5.7% and 8% prevalence in frozen fish collected from restaurant cold storage and fresh fish collected immediately from the landing site. Antimicrobial susceptibility test of\n                      <jats:italic>E. coli<\/jats:italic>\n                      O157:H7 isolates for kanamycin (30\u2009ug), tetracycline (30\u2009ug), cefoxitin (30\u2009ug), erythromycin (20\u2009ug), streptomycin (10\u2009ug), sulfamethoxazole (25\u2009ug), and ampicillin (10\u2009ug) showed resistance to most antibiotics. The results indicate that\n                      <jats:italic>E. coli<\/jats:italic>\n                      O157:H7 was isolated from 10 samples from fish and fish handling equipment in this study. In addition, isolates were found resistant to penicillin and ampicillin. Kanamycin, tetracycline, and cefoxitin were found effective in inhibiting the growth of all of the isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The presence of\n                      <jats:italic>E. coli<\/jats:italic>\n                      O157:H7 isolates and its resistance to drugs pose significant economic and public health challenges. Thus, it requires implementing interventions like freezing and proper fish handling practices to minimize the level of contamination of fish in Ethiopia.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/bmri\/5097078","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T07:36:25Z","timestamp":1781076985000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence and Antimicrobial Susceptibility Profile of\n                    <i>E. coli<\/i>\n                    O157:H7 in Fish Collected From Lake Ziway, Ethiopia"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6042-7089","authenticated-orcid":false,"given":"Assaye Desta","family":"Amare","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4457-1289","authenticated-orcid":false,"given":"Fufa Abunna","family":"Kurra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fikru Regassa","family":"Gari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,10]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e02996"},{"key":"e_1_2_13_2_2","volume-title":"Food and Agricultural Organization of the United Nations","author":"Food and agricultural organization (FAO)","year":"2012"},{"key":"e_1_2_13_3_2","volume-title":"Challenges of Sustainable Livelihood: The Case of Fishing Communities Around Lake Chamo","author":"Shado T.","year":"2006"},{"key":"e_1_2_13_4_2","unstructured":"ISO ISO 6887-1:2017 Microbiology of the Food Chain\u2014Preparation of Test Samples Initial Suspension and Decimal Dilutions for Microbiological Examination\u2014Part 1 General Rules for the Preparation of the Initial Suspension and Decimal Dilutions (ISO 6887-1: 2017). 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