{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T09:04:58Z","timestamp":1789722298937,"version":"4.0.1"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:00:00Z","timestamp":1774396800000},"content-version":"vor","delay-in-days":83,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Schistosomiasis remains a major public health concern in Benin. Freshwater snails of the genus\n                    <jats:italic>Bulinus<\/jats:italic>\n                    serve as intermediate hosts for schistosomes, trematode parasites responsible for bilharzia. The urinary form, caused by\n                    <jats:italic>Schistosoma haematobium<\/jats:italic>\n                    , is the most prevalent and is primarily transmitted by\n                    <jats:italic>Bulinus truncatus<\/jats:italic>\n                    and\n                    <jats:italic>Bulinus globosus<\/jats:italic>\n                    , whereas the intestinal form, caused by\n                    <jats:italic>Schistosoma mansoni<\/jats:italic>\n                    , is transmitted by\n                    <jats:italic>Bulinus forskalii<\/jats:italic>\n                    . Effective control strategies require an understanding of the molecular diversity and genetic structure of\n                    <jats:italic>Bulinus<\/jats:italic>\n                    populations.\n                    <jats:italic>Bulinus<\/jats:italic>\n                    samples were collected from six localities in southern Benin: S\u00f4\u2010Ava, Azowliss\u00e8, Aplahou\u00e9, Kpinnou, Hou\u00e9yogb\u00e9, and Za\u2010Kpota. Samples were preserved in absolute alcohol. DNA was extracted, and fragments of the\n                    <jats:italic>Cox1<\/jats:italic>\n                    and\n                    <jats:italic>SSU<\/jats:italic>\n                    genes were amplified. Molecular diversity was assessed using PCR\u2010RFLP, with amplified products digested using\n                    <jats:italic>HaeIII<\/jats:italic>\n                    ,\n                    <jats:italic>HinfI<\/jats:italic>\n                    , and\n                    <jats:italic>DdeI<\/jats:italic>\n                    restriction enzymes. Molecular variance analysis (AMOVA) was performed to evaluate population structure. AMOVA revealed heterozygosity deficits in\n                    <jats:italic>Bulinus<\/jats:italic>\n                    populations from all six localities and deviations from panmixia in S\u00f4\u2010Ava, Aplahou\u00e9, Hou\u00e9yogb\u00e9, Kpinnou, and Za\u2010Kpota. Enzymatic digestion indicated monomorphism for\n                    <jats:italic>B. globosus<\/jats:italic>\n                    in Za\u2010Kpota and Hou\u00e9yogb\u00e9 with the\n                    <jats:italic>SSU<\/jats:italic>\n                    gene and for\n                    <jats:italic>B. truncatus<\/jats:italic>\n                    in S\u00f4\u2010Ava and Azowliss\u00e8 with the\n                    <jats:italic>Cox1<\/jats:italic>\n                    gene. Conversely, molecular polymorphism was observed for\n                    <jats:italic>B. globosus<\/jats:italic>\n                    in S\u00f4\u2010Ava and Aplahou\u00e9 with the C\n                    <jats:italic>ox1<\/jats:italic>\n                    gene, whereas\n                    <jats:italic>B. truncatus<\/jats:italic>\n                    in S\u00f4\u2010Ava and Azowliss\u00e8 showed identical\n                    <jats:italic>SSU<\/jats:italic>\n                    gene profiles. These findings provide preliminary insights into the molecular diversity and genetic structure of\n                    <jats:italic>Bulinus<\/jats:italic>\n                    populations in southern Benin. The observed genetic variability and structure have implications for understanding the transmission dynamics of\n                    <jats:italic>S. haematobium<\/jats:italic>\n                    and for designing targeted schistosomiasis control strategies.\n                  <\/jats:p>","DOI":"10.1155\/bmri\/5536391","type":"journal-article","created":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T11:05:02Z","timestamp":1774436702000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Diversity and Ecological Distribution of\n                    <i>Bulinus<\/i>\n                    Populations in Southern Benin Using PCR\u2010RFLP Analysis"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9743-2699","authenticated-orcid":false,"given":"Elokou","family":"Alabi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aboudou Chabi","family":"Do","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Nougbode","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halfane","family":"Lehmane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haziz","family":"Sina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulin","family":"Sedah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adolphe","family":"Adjanohoun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Sezonlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8160-5954","authenticated-orcid":false,"given":"Lamine","family":"Baba-Moussa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,25]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000412"},{"key":"e_1_2_11_2_2","volume-title":"Infection Prevention and Control During Health Care When Coronavirus Disease (COVID-19) Is Suspected or Confirmed: Interim Guidance","author":"World Health Organization","year":"2021"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9002(00)00334-X"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182001008046"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-016-5152-9"},{"key":"e_1_2_11_6_2","first-page":"33","article-title":"A Review of Intestinal Helminthiasis in Nigeria and the Need for School-Based Intervention","volume":"6","author":"Ekundayo O. 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