{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T04:05:00Z","timestamp":1784606700727,"version":"3.55.0"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"vor","delay-in-days":200,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020880","name":"Universidade Federal do Maranh\u00e3o","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020880","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["445615\/2024-9"],"award-info":[{"award-number":["445615\/2024-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003758","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e ao Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico do Maranh\u00e3o","doi-asserted-by":"publisher","award":["IECT-02885\/17"],"award-info":[{"award-number":["IECT-02885\/17"]}],"id":[{"id":"10.13039\/501100003758","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background\/Objectives<\/jats:title>\n                    <jats:p>\n                      The study evaluated the in vitro antibacterial activity of the ethanolic extract of\n                      <jats:italic>Anacardium occidentale<\/jats:italic>\n                      flowers (EHAo) against ESKAPE pathogens and investigated the in vivo effects of the extract in\n                      <jats:italic>Tenebrio molitor<\/jats:italic>\n                      lethally infected with\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      . In silico analyses investigated the affinity of compounds with sortase A.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Chemical composition was characterized by HPLC and LC\u2010MS. Antibacterial activity was determined by minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and assays of adhesion and biofilm formation using\n                      <jats:italic>S. aureus<\/jats:italic>\n                      ,\n                      <jats:italic>Enterococcus faecalis<\/jats:italic>\n                      ,\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      ,\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      , and clinical MRSA strains. In vivo toxicity and antimicrobial efficacy were assessed in T. molitor larvae infected with\n                      <jats:italic>S. aureus<\/jats:italic>\n                      (10\n                      <jats:sup>5<\/jats:sup>\n                      CFU\/mL) and treated with PBS, ciprofloxacin (1.56\n                      <jats:italic>\u03bc<\/jats:italic>\n                      g\/mL), or EHAo (3.12\u201312.5 mg\/mL). Molecular docking evaluated the interactions between the chemical compounds identified in EHAo and the sortase A active site.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      EHAo contained shikimic acid, gallic acid, quercetin, isoquercetin, galloyl\u2010glucose, digalloyl\u2010glucose, and tetragalloylglucose isomers. The extract exhibited bactericidal activity (MIC 1.25\u20136.25 mg\/mL), with the strongest effects against\n                      <jats:italic>S. aureus<\/jats:italic>\n                      , MRSA, and E. faecalis. EHAo inhibited adhesion and biofilm formation, except in\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      . The extract showed low toxicity in vivo and was efficient in improving the larvae\u2032s survival following lethal infection with\n                      <jats:italic>S. aureus<\/jats:italic>\n                      . All compounds showed affinity to sortase A active site. However, digalloyl\u2010glucose displayed the highest predicted affinity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      EHAo reduces bacterial virulence and improves survival in\n                      <jats:italic>S. aureus<\/jats:italic>\n                      \u2013infected larvae, an activity probably related to the bactericidal activity, inhibition of adhesion and biofilm formation, and interaction with the sortase A active site.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/bmri\/6579435","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T03:51:36Z","timestamp":1784605896000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anti\u2010Infective Effect of\n                    <i>Anacardium occidentale<\/i>\n                    L. Flowers Against Eskape Group Bacteria In Vitro, In Vivo, and In Silico"],"prefix":"10.1155","volume":"2026","author":[{"given":"P\u00e2mela Gomes","family":"Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josivan Regis","family":"Farias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8469-4315","authenticated-orcid":false,"given":"Simone Batista","family":"Muniz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle Cristine Gomes","family":"Franco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariadina Jansen Campos","family":"Fontes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liane Maria Rodrigues","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia Raquel Fernandes","family":"Nascimento","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3578-1869","authenticated-orcid":false,"given":"Claudia Quintino","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra Martins","family":"dos Santos Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9865-9262","authenticated-orcid":false,"given":"Rosane Nassar Meireles","family":"Guerra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,20]]},"reference":[{"key":"e_1_2_15_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00181-19"},{"key":"e_1_2_15_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c00110"},{"key":"e_1_2_15_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-024-01054-w"},{"key":"e_1_2_15_4_2","first-page":"49","article-title":"Hospital Infections Related to Invasive Procedures in Intensive Care Units: A Literature Review","volume":"3","author":"Sousa M.","year":"2017","journal-title":"Journal of Infection Prevention and Health"},{"key":"e_1_2_15_5_2","doi-asserted-by":"publisher","DOI":"10.25248\/reas.e7319.2021"},{"key":"e_1_2_15_6_2","doi-asserted-by":"publisher","DOI":"10.33448\/rsd-v11i15.37997"},{"key":"e_1_2_15_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202206602"},{"key":"e_1_2_15_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13050400"},{"key":"e_1_2_15_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11020424"},{"key":"e_1_2_15_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.12167"},{"key":"e_1_2_15_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2019.10.008"},{"key":"e_1_2_15_12_2","volume-title":"National Guideline for the Elaboration of an Antimicrobial Management Program in Health Services","author":"Ag\u00eancia Nacional de Vigil\u00e2ncia Sanit\u00e1ria","year":"2023"},{"key":"e_1_2_15_13_2","doi-asserted-by":"publisher","DOI":"10.2174\/1568026623666230417105227"},{"key":"e_1_2_15_14_2","doi-asserted-by":"publisher","DOI":"10.4236\/jbm.2016.44012"},{"key":"e_1_2_15_15_2","first-page":"123","article-title":"Ethnobotanical Study of the Cashew Tree (Anacardium occidentale L.): A Natural Tree From Brazil","volume":"29","author":"Freire J. C. P.","year":"2017","journal-title":"Revista UNING\u00c1 Review"},{"key":"e_1_2_15_16_2","doi-asserted-by":"publisher","DOI":"10.7198\/geintec.v8i1.1121"},{"key":"e_1_2_15_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2017.09.030"},{"key":"e_1_2_15_18_2","doi-asserted-by":"publisher","DOI":"10.33448\/rsd-v9i8.6459"},{"key":"e_1_2_15_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.03.014"},{"key":"e_1_2_15_20_2","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing. Twenty-Second Informational Supplement Clinical and Laboratory Standards Institute","author":"Clinical and Laboratory Standards Institute","year":"2015"},{"key":"e_1_2_15_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0232987"},{"key":"e_1_2_15_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jscs.2011.03.016"},{"key":"e_1_2_15_23_2","doi-asserted-by":"publisher","DOI":"10.14808\/sci.plena.2023.044501"},{"key":"e_1_2_15_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/ph13030046"},{"key":"e_1_2_15_25_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24032296"},{"key":"e_1_2_15_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2015.10.004"},{"key":"e_1_2_15_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2016.01.002"},{"key":"e_1_2_15_28_2","doi-asserted-by":"crossref","unstructured":"Bitencourt-FerreiraG.andAzevedoW. F. Molegro Virtual Docker for Docking Docking Screens for Drug Discovery2019 Springer https:\/\/doi.org\/10.1007\/978-1-4939-9752-7_10.","DOI":"10.1007\/978-1-4939-9752-7_10"},{"key":"e_1_2_15_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/1758-2946-4-17"},{"key":"e_1_2_15_30_2","volume-title":"SAS, User\u2032s Guide: Statistics. Version 9.0","author":"SAS Institute Inc","year":"2002"},{"key":"e_1_2_15_31_2","doi-asserted-by":"publisher","DOI":"10.33448\/rsd-v9i8.5802"},{"key":"e_1_2_15_32_2","first-page":"121","article-title":"Tannins From Cashew Bark: Antimicrobial Activity","volume":"36","author":"Pereira A. V.","year":"2015","journal-title":"Journal of Agroindustry and Technology"},{"key":"e_1_2_15_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-024-05355-3"},{"key":"e_1_2_15_34_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10020188"},{"key":"e_1_2_15_35_2","first-page":"867","article-title":"Anticomplement and Antimicrobial Activities of Flavonoids from Entada Phaseoloides","volume":"7","author":"Li K.","year":"2012","journal-title":"Natural Products Communications"},{"key":"e_1_2_15_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crmicr.2022.100171"},{"key":"e_1_2_15_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcma.2017.07.012"},{"key":"e_1_2_15_38_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13070623"},{"key":"e_1_2_15_39_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2017.1313372"},{"key":"e_1_2_15_40_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22136890"},{"key":"e_1_2_15_41_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9060314"},{"key":"e_1_2_15_42_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29071528"},{"key":"e_1_2_15_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e17657"},{"key":"e_1_2_15_44_2","doi-asserted-by":"publisher","DOI":"10.1080\/20477724.2023.2285187"},{"key":"e_1_2_15_45_2","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1605.05005"},{"key":"e_1_2_15_46_2","doi-asserted-by":"publisher","DOI":"10.5935\/0103-507X.20220235-en"},{"key":"e_1_2_15_47_2","first-page":"9","article-title":"Effect of Cashew (Anacardium occidentale L.) Peduncle Bagasse Extract on Streptococcus mutans and Its Biofilm. Braz","volume":"2","author":"Furtado M. A. M.","year":"2014","journal-title":"Brazilian Journal of Biosciences"},{"key":"e_1_2_15_48_2","doi-asserted-by":"publisher","DOI":"10.1166\/jctn.2020.9270"},{"key":"e_1_2_15_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.PSIB-0004-2018"},{"key":"e_1_2_15_50_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18102217"},{"key":"e_1_2_15_51_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28010158"},{"key":"e_1_2_15_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matpr.2020.01.551"},{"key":"e_1_2_15_53_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27082494"},{"key":"e_1_2_15_54_2","doi-asserted-by":"publisher","DOI":"10.4103\/2221-1691.314044"},{"key":"e_1_2_15_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-68279-w"},{"key":"e_1_2_15_56_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13081001"},{"key":"e_1_2_15_57_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22168995"},{"key":"e_1_2_15_58_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11121834"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/bmri\/6579435","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/bmri\/6579435","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/bmri\/6579435","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T03:51:40Z","timestamp":1784605900000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/bmri\/6579435"}},"subtitle":[],"editor":[{"given":"Poorani","family":"Gurumallesh Prabu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":58,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/bmri\/6579435"],"URL":"https:\/\/doi.org\/10.1155\/bmri\/6579435","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-01-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"6579435"}}